转GmGT-2B基因大豆的耐盐性分析

发布时间：2018-08-26 12:41

【摘要】：采用盆栽试验方法,苗期在盐胁迫条件下,比较GmGT-2B转基因大豆与非转基因大豆的损伤程度、超氧化物歧化酶(SOD)活性,丙二醛(MDA)、脯氨酸和K~+、Na~+含量以及基因表达量变化差异。结果表明:盐胁迫对转基因大豆叶片的伤害程度明显低于对照;SOD活性、脯氨酸含量、丙二醛(MDA)含量在盐胁迫5 d后均与对照有显著或极显著差异;植株的Na~+含量随盐胁迫时间的增加而升高,在胁迫第8天转基因大豆的Na~+含量达到对照约2倍;转基因大豆的基因的表达量呈上调趋势,在第8天达到表达高峰。基因表达量的变化趋势与生理指标SOD活性、脯氨酸含量和Na~+含量表现的一致。结果表明GmGT-2B基因与盐应答反应有关,其中转GmGT-2B大豆株系N11和N24盐害程度较低,该基因和转GmGT-2B基因大豆具有较大的应用价值。
[Abstract]:The damage degree, superoxide dismutase (SOD) activity, malondialdehyde (MDA),) proline, K ~ (+) Na ~ + and gene expression of GmGT-2B transgenic soybean and non-transgenic soybean were compared under salt stress at seedling stage by pot experiment. The results showed that the damage degree of salt stress to transgenic soybean leaves was significantly lower than that of the control. The contents of proline and malondialdehyde (MDA) were significantly different from those of the control after 5 days of salt stress. The Na~ content of transgenic soybean increased with the increase of salt stress time, and the Na~ content of transgenic soybean was about 2 times that of control on the 8th day of stress, and the gene expression of transgenic soybean showed an upward trend, and reached the peak on the 8th day. The trend of gene expression was consistent with that of SOD activity, proline content and Na~ content. The results showed that the GmGT-2B gene was related to salt response, and the transgenic GmGT-2B soybean lines N11 and N24 had lower salt damage. The GmGT-2B gene and the transgenic GmGT-2B gene soybean had great application value.
【作者单位】：东北农业大学大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室;
【基金】：抗逆转基因大豆新品种培育专项(2014ZX08004-002) 黑龙江省教育厅一般项目(10531012)
【分类号】：S565.1

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