弥漫性大B细胞淋巴瘤组织中Bcl-6基因异常情况及其临床意义

发布时间：2018-08-27 11:41

【摘要】：目的观察弥漫性大B细胞淋巴瘤(DLBCL)中Bcl-6基因异常情况,并分析其与临床病理特征的关系。方法 DLBCL患者42例,采用Bcl-6双色断裂点分离探针荧光原位杂交技术检测Bcl-6基因,采用免疫组织化学方法检测Bcl-6蛋白表达,采用χ2检验分析Bcl-6基因异常与DLBCL临床病理特征的关系。结果 42例DLBCL患者中发生Bcl-6基因易位6例,发生Bcl-6基因扩增3例,Bcl-6蛋白表达阳性19例。Bcl-6基因易位患者Bcl-6蛋白表达阳性率较无易位者高(P=0.014);Bcl-6基因是否易位患者DLBCL分型、性别、年龄比较差异均无统计学意义(P均0.05)。Bcl-6基因是否扩增患者Bcl-6蛋白表达、DLBCL分型、性别、年龄比较差异均无统计学意义(P均0.05)。结论 DLBCL中Bcl-6基因存在易位和扩增两种情况,并且Bcl-6基因易位与患者Bcl-6蛋白表达有密切的联系。
[Abstract]:Objective to observe the abnormality of Bcl-6 gene in (DLBCL) of diffuse large B cell lymphoma and to analyze its relationship with clinicopathological features. Methods in 42 patients with DLBCL, the Bcl-6 gene was detected by fluorescence in situ hybridization (Fish) with Bcl-6 double color break point separation probe, the expression of Bcl-6 protein was detected by immunohistochemical method, and the relationship between Bcl-6 gene abnormality and the clinicopathological characteristics of DLBCL was analyzed by 蠂 2 test. Results there were 6 cases of Bcl-6 gene translocation in 42 patients with DLBCL, and 19 cases of positive expression of Bcl-6 protein in 3 cases of Bcl-6 gene amplification. The positive rate of Bcl-6 protein expression in patients with Bcl-6 gene translocation was higher than that in patients without translocation (P0. 014). There was no significant difference in age (P0. 05). Whether the Bcl-6 gene was amplified or not, there was no significant difference in the expression of Bcl-6 protein and DLBCL type (P 0. 05). There was no significant difference in sex and age (all P 0. 05). Conclusion there are two kinds of translocation and amplification of Bcl-6 gene in DLBCL, and the translocation of Bcl-6 gene is closely related to the expression of Bcl-6 protein in patients.
【作者单位】：潍坊医学院;潍坊市人民医院;
【基金】：山东省潍坊市科学技术发展计划项目(20100220)
【分类号】：R733.1

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