热补针法对风寒湿阻型类风湿关节炎患者血浆中能量代谢相关基因Atp 5O、Atp 6V1B2表达的影响
发布时间：2018-08-27 12:00
【摘要】：目的:观察热补针法对风寒湿阻型类风湿关节炎(RA)患者中医证候积分及血浆中能量代谢相关基因ATP合成酶亚单位O(Atp 5O)、溶酶体V1亚单位B 2(Atp 6V1B2)表达的影响,探讨热补针法"取热"的分子生物学机制。方法:将符合纳入标准的60例风寒湿阻型RA患者随机分为热补针法组、对照组,每组30例。两组均取关元、气海、足三里和关节局部腧穴,热补针法组施以热补针法,对照组施以平补平泻法,1次/d,连续5次为1个疗程,共治疗2个疗程。同时招募30名健康体检者作为正常对照组。观察患者治疗前后中医证候积分变化,RT-PCR法检测正常人及患者外周血中Atp 5O、Atp 6V1B2 mRNA的表达。结果:治疗后两组患者中医证候积分均较治疗前明显降低(P0.05),热补针法组证候积分降低较对照组明显(P0.05)。治疗前RA患者外周血中Atp 5O、Atp 6V1B2 mRNA表达均较正常人降低(P0.05);治疗后两组患者Atp 5O、Atp 6V1B2 mRNA表达均较治疗前明显上调(P0.05),且热补针法组的上调明显高于对照组(P0.05),两组患者Atp 5O、Atp 6V1B2 mRNA表达与正常对照组的差异无统计学意义(P0.05)。结论:热补针法上调血浆中能量代谢相关基因Atp 5O、Atp 6V1B2的表达是改善风寒湿阻型RA患者症状和"取热"的分子机制之一。
[Abstract]:Methods: 60 RA patients with wind-cold and dampness resistance were randomly divided into heat supplement acupuncture group and control group with 30 cases in each group. Guanyuan, Qihai, Zusanli and joint local acupoints were taken in both groups. Heat supplement acupuncture was used in the heat supplement acupuncture group, and the control group was treated with the flat tonifying and flattening and reducing method once a day, for 5 consecutive times as a course of treatment for 2 courses of treatment. At the same time, 30 healthy people were recruited as the normal control group. The changes of TCM syndromes before and after treatment were observed. RT-PCR was used to detect the expression of Atp 5 OG Atp 6V1B2 mRNA in the peripheral blood of normal subjects and patients. Results: after treatment, the scores of TCM syndromes in the two groups were significantly lower than those before the treatment (P0.05), and the scores of syndrome in the heat supplement acupuncture group were significantly lower than those in the control group (P0.05). Before treatment, the expression of Atp 5OO P 6V1B2 mRNA in peripheral blood of RA patients was significantly lower than that of normal controls (P0.05), and the expression of Atp 5OO Atp 6V1B2 mRNA in both groups was significantly higher than that before treatment (P0.05), and the up-regulation of Atp 5OO Atp 6V1B2 mRNA in heat supplementation group was significantly higher than that in control group (P0.05), and the Atp 5OU Atp expression in both groups was significantly higher than that in control group (P0.05). There was no significant difference between 6V1B2 mRNA expression and normal control group (P0.05). Conclusion: the up-regulation of the expression of Atp _ 5OO _ (Atp) 6V1B2 is one of the molecular mechanisms for improving symptoms and "heat removal" in patients with RA with wind-cold and dampness resistance.
【作者单位】： 甘肃中医药大学医学技术学院;兰州大学第二医院中医科;甘肃中医药大学针灸推拿学院;甘肃中医药大学附属医院风湿骨病科;
【基金】：甘肃省自然科学基金项目(No.1208RJZA 185) 国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(No.2305135901)
【分类号】：R246.1
[Abstract]:Methods: 60 RA patients with wind-cold and dampness resistance were randomly divided into heat supplement acupuncture group and control group with 30 cases in each group. Guanyuan, Qihai, Zusanli and joint local acupoints were taken in both groups. Heat supplement acupuncture was used in the heat supplement acupuncture group, and the control group was treated with the flat tonifying and flattening and reducing method once a day, for 5 consecutive times as a course of treatment for 2 courses of treatment. At the same time, 30 healthy people were recruited as the normal control group. The changes of TCM syndromes before and after treatment were observed. RT-PCR was used to detect the expression of Atp 5 OG Atp 6V1B2 mRNA in the peripheral blood of normal subjects and patients. Results: after treatment, the scores of TCM syndromes in the two groups were significantly lower than those before the treatment (P0.05), and the scores of syndrome in the heat supplement acupuncture group were significantly lower than those in the control group (P0.05). Before treatment, the expression of Atp 5OO P 6V1B2 mRNA in peripheral blood of RA patients was significantly lower than that of normal controls (P0.05), and the expression of Atp 5OO Atp 6V1B2 mRNA in both groups was significantly higher than that before treatment (P0.05), and the up-regulation of Atp 5OO Atp 6V1B2 mRNA in heat supplementation group was significantly higher than that in control group (P0.05), and the Atp 5OU Atp expression in both groups was significantly higher than that in control group (P0.05). There was no significant difference between 6V1B2 mRNA expression and normal control group (P0.05). Conclusion: the up-regulation of the expression of Atp _ 5OO _ (Atp) 6V1B2 is one of the molecular mechanisms for improving symptoms and "heat removal" in patients with RA with wind-cold and dampness resistance.
【作者单位】： 甘肃中医药大学医学技术学院;兰州大学第二医院中医科;甘肃中医药大学针灸推拿学院;甘肃中医药大学附属医院风湿骨病科;
【基金】：甘肃省自然科学基金项目(No.1208RJZA 185) 国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(No.2305135901)
【分类号】：R246.1
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