地黄属核基因与叶绿体基因DNA条形码比较研究

发布时间：2018-08-27 17:25

【摘要】：目的比较单拷贝核基因、叶绿体基因及ITS等DNA条形码在地黄属的物种分辨率,探讨地黄属种间亲缘关系。方法利用距离法与建树法分析了地黄属植物13个居群的12个单拷贝核基因、6个叶绿体基因以及ITS片段物种鉴定成功率,构建了地黄属系统发育树。结果叶绿体matk和rbc L、单拷贝核基因物种分辨率普遍较低,多基因联合分析有助于物种鉴定成功率的提高。在NJ树中,地黄-茄叶地黄聚为一支,其他物种都是单系群,裂叶地黄位于系统发育树基部。结论 ITS是地黄属物种鉴定的核心条形码,psb A-trnH、trnL-F、trnM-V、trnS-G可以作为备选方案,核基因RR255与R257可用作地黄专用鉴定条形码。四倍体地黄起源方式有待进一步澄清。
[Abstract]:Objective to compare the species resolution of single copy nuclear genes, chloroplast genes and ITS DNA barcodes in the genus Rehmannia, and to explore the relationship between species of Rehmannia. Methods A phylogenetic tree of the genus Rehmannia was constructed by using distance method and tree building method to analyze 12 single copy nuclear genes, 6 chloroplast genes and ITS fragments of 13 populations of Rehmannia. Results the species resolution of chloroplast matk and rbc L, single copy nuclear gene was generally low, and the combined analysis of polygenes was helpful to improve the success rate of species identification. In the NJ tree, Rehmannia glutinosa and Rehmannia solanica were clustered into one branch, and the other species were monophyletic groups, and Dihuang was located at the base of the phylogenetic tree. Conclusion ITS is the core bar code for species identification of Rehmannia. PSB A-trnHtrnL-FtrnM-VftrnS-G can be used as an alternative scheme, and nuclear genes RR255 and R257 can be used as specific bar codes for the identification of Rehmannia glutinosa. The origin of tetraploid Rehmannia glutinosa needs further clarification.
【作者单位】：西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室;
【基金】：国家自然科学基金项目(31670219,31370353) 陕西省自然科学基础研究计划(2015JM3106) 陕西省教育厅项目(11JS093)
【分类号】：Q943.2

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