基于HRM体系开发抗稻瘟病基因Pi2特异性分子标记

发布时间：2018-08-29 14:08

【摘要】：Pi2是一个具有广谱抗性的稻瘟病抗性基因,利用分子标记辅助选择技术选育含有Pi2抗性基因的水稻品种已得到越来越广泛的应用。本研究根据稻瘟病抗性基因Pi2及其复等位基因的序列,利用HRM技术开发与Pi2完全共分离的分子标记,并结合水稻DNA快速提取方法,建立中高通量辅助选择体系。该方法体系准确高效、简便快速、成本低廉,能够大大提高抗病育种选育的效率,为分子标记辅助选择育种或者水稻种质资源Pi2基因型鉴定提供技术选择。
[Abstract]:Pi2 is a broad-spectrum resistant rice blast resistance gene. The selection of rice varieties with Pi2 resistance gene by molecular marker-assisted selection technology has been more and more widely used. Based on the sequence of rice blast resistance gene Pi2 and its complex alleles, a molecular marker completely separated from Pi2 was developed by using HRM technique, and a medium and high throughput assisted selection system was established by combining with the rapid extraction method of DNA in rice (Oryza sativa L.). The method is accurate, efficient, simple, rapid and low cost. It can greatly improve the efficiency of breeding for disease resistance, and provide a technical choice for molecular marker-assisted selection or Pi2 genotyping of rice germplasm resources.
【作者单位】：华南农业大学生命科学学院;深圳市作物分子设计育种研究院深圳市分子设计育种重点实验室;
【基金】：国家自然科学基金青年基金项目(31500254) 广东省引进创新科研团队计划资助项目(No.201001S010472-5509) 深圳市孔雀计划引进团队计划资助项目(KQF201109160004A) 深圳市技术攻关项目(JSGG20150508105340526) 深圳市农业技术成果转化项目(NYKJ20140922151049440)共同资助
【分类号】：S435.111.41

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