蜻蜓凤梨AfGAI基因的克
发布时间：2018-08-29 16:53
【摘要】：根据蜻蜓凤梨转录组信息,通过RACE技术(rapid amplification of cDNA ends,RACE)获得蜻蜓凤梨中一个DELLA蛋白的cDNA序列,并将其命名为AfGAI。AfGAI cDNA全长1 960 bp,开放阅读框为1 764 bp,编码587个氨基酸。氨基酸序列分析结果显示,AfGAI蛋白含有DELLA蛋白家族共有的功能结构域:DELLA结构域和GRAS结构域,与菠萝(Ananas comosus)、海枣(Phoenix dactylifera)、油棕(Elaeis guineensis)、巨桉(Eucalyptus grandis)、陆地棉(Gossypium hirsutum)中的GAI同源性分别为90%、73%、73%、65%、59%。进一步构建了35S:AfGAI过表达载体,为解析凤梨科植物开花机理提供理论依据。
[Abstract]:According to the transcriptome information of dragonfly and pineapple, the cDNA sequence of a DELLA protein in dragonfly and pineapple was obtained by rapid amplification of cDNA ends (RACE). The full length of the DELLA protein was 1 960 bp, the open reading frame was 1 764 BP and 587 amino acids were encoded. The amino acid sequence analysis showed that the AFGAI protein contained DELLA eggs. The common functional domains of the Bai family are DELLA and GRAS domains, which are 90%, 73%, 73%, 65% and 59% homologous to GAI in pineapple (Ananas comosus), jujube (Phoenix dactylifera), oil palm (Elaeis guineensis), Eucalyptus grandis, and upland cotton (Gossypium hirsutum). The 35S: AfGAI overexpression vector was constructed to understand the above-mentioned genes. To provide theoretical basis for flowering mechanism of pineapple plants.
【作者单位】： 海南大学农学院;中国热带农业科学院热带作物品种资源研究所农业部华南热带作物基因资源与种质创制重点开放实验室海南省热带作物遗传改良与创新重点实验室;
【基金】：国家自然基金面上项目(31372106);国家自然基金青年科学基金项目(31601793) 海南省自然科学基金面上项目(20163127)共同资助
【分类号】：Q943.2;S682.3
本文编号：2211821
[Abstract]:According to the transcriptome information of dragonfly and pineapple, the cDNA sequence of a DELLA protein in dragonfly and pineapple was obtained by rapid amplification of cDNA ends (RACE). The full length of the DELLA protein was 1 960 bp, the open reading frame was 1 764 BP and 587 amino acids were encoded. The amino acid sequence analysis showed that the AFGAI protein contained DELLA eggs. The common functional domains of the Bai family are DELLA and GRAS domains, which are 90%, 73%, 73%, 65% and 59% homologous to GAI in pineapple (Ananas comosus), jujube (Phoenix dactylifera), oil palm (Elaeis guineensis), Eucalyptus grandis, and upland cotton (Gossypium hirsutum). The 35S: AfGAI overexpression vector was constructed to understand the above-mentioned genes. To provide theoretical basis for flowering mechanism of pineapple plants.
【作者单位】： 海南大学农学院;中国热带农业科学院热带作物品种资源研究所农业部华南热带作物基因资源与种质创制重点开放实验室海南省热带作物遗传改良与创新重点实验室;
【基金】：国家自然基金面上项目(31372106);国家自然基金青年科学基金项目(31601793) 海南省自然科学基金面上项目(20163127)共同资助
【分类号】：Q943.2;S682.3
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1 丛汉卿;乙烯诱导蜻蜓凤梨开花相关基因表达分析及其催花分子机制的初步研究[D];海南大学;2012年
，本文编号：2211821
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