甘蔗ScF-box基因cDNA全长克隆和生物信息学分析

发布时间：2018-08-31 14:20

【摘要】：【目的】独脚金内酯是近年来发现的一种新型激素,能够有效抑制植物分枝(蘖),在其信号转导途径中MAX2/RMS4/D3基因扮演着十分关键的作用。为了研究该基因在甘蔗分蘖性状表现中的功能作用具有重要意义。【方法】本研究利用RT-PCR和RACE技术克隆出其同源基因(ScF-box)的c DNA全长,并对其序列和编码蛋白开展生物信息学分析。【结果】ScF-box基因的c DNA全长为2642 bp(KR870233),具2103 bp的开放阅读框(ORF,113~2215 bp),编码700个氨基酸;蛋白质分析表明,ScF-box为不稳定的水溶性非分泌蛋白,主要在氨基酸生物合成和中央中介代谢中发挥作用。亚细胞定位于细胞质,存在22个磷酸化位点,4个NES信号和1个NLS信号位点,不存在信号肽,二级结构以α螺旋和无规则卷曲为主,保守结构域和三级结构预测均表明该蛋白存在一个F-box结构,能特异识别与之作用的底物。【结论】推测ScF-box可能作为独脚金内酯调控甘蔗分蘖信号转导途径中一个具有特异性识别功能的组分而起作用。进化树分析表明该蛋白与高粱、玉米等单子叶植物进化关系较近。
[Abstract]:[OBJECTIVE] Dipterygium lactone is a new hormone discovered in recent years, which can effectively inhibit plant branching (tillering). The MAX2/RMS4/D3 gene plays a very important role in signal transduction pathway. In order to study the function of the gene in the performance of sugarcane tillering traits, it is important to use RT-PCR in this study. The full length of the ScF-box gene was cloned by RACE and its sequence and coding protein were analyzed by bioinformatics. [Results] The full length of the ScF-box gene was 2642 BP (KR870233), with an open reading frame of 2103 BP (ORF, 113-2215 bp), encoding 700 amino acids. Sexual nonsecretory proteins, mainly involved in amino acid biosynthesis and central mediated metabolism, are subcellular localized in the cytoplasm with 22 phosphorylation sites, 4 NES signaling sites and 1 NLS signaling site. There is no signal peptide. The secondary structure of the protein is mainly alpha helix and irregular curl. Conserved domain and tertiary structure prediction indicate that the protein is mainly composed of a helix and irregular curl. [Conclusion] It is speculated that ScF-box may play a role as a specific recognition component in sugarcane tillering signal transduction pathway regulated by monocotyledons such as sorghum and maize.
【作者单位】：云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室;云南农业大学农学与生物技术学院;
【基金】：国家自然科学基金(31360359,31660421) 云南省中青年学术技术带头人后备人才(2014HB038)
【分类号】：S566.1

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