肉鸡MSMO1基因的克隆及表达模式分析
发布时间：2018-09-03 09:59
【摘要】：【目的】克隆肉鸡MSMO1(Methylsterol monooxygenase 1)基因序列,并分析其表达模式,为研究MSMO1基因的功能奠定基础。【方法】利用RT-PCR技术克隆肉鸡MSMO1基因,采用生物信息学方法分析其蛋白质结构,并用定量PCR技术检测MSMO1基因在组织间的表达差异。【结果】肉鸡MSMO1基因的c DNA序列长度为1404 bp,编码296个氨基酸;MSMO1蛋白145~274位氨基酸序列残基存在FA-hydroxylase结构域,为亲水蛋白。进化树分析表明,肉鸡MSMO1氨基酸序列与日本鹌鹑和鹦鹉的MSMO1分子亲缘关系较近;二级结构主要以α-螺旋(41.89%)为主。组织表达水平结果显示,在8种组织中均检测到MSMO1基因的表达,且在肝脏中的表达水平极显著高于其它7种组织中的表达水平(P0.01),在脾脏和胸肌中的表达水平极显著低于在肺脏和肝脏中的表达水平(P0.01)。【结论】本结果将为进一步研究MSMO1基因的结构和功能奠定基础。
[Abstract]:[objective] to clone the MSMO1 (Methylsterol monooxygenase 1) gene sequence and analyze its expression pattern. [methods] the MSMO1 gene was cloned by RT-PCR technique and its protein structure was analyzed by bioinformatics. Quantitative PCR technique was used to detect the difference of the expression of MSMO1 gene among tissues. [results] the c DNA sequence of MSMO1 gene of broiler chicken was 1404 bp, encoding 296amino acid sequence residues of MSMO1 protein 145N 274, which existed in FA-hydroxylase domain and was hydrophilic protein. The phylogenetic tree analysis showed that the amino acid sequence of MSMO1 was closely related to the MSMO1 of Japanese quail and parrot, and the secondary structure was mainly 伪 -helix (41.89%). The results of tissue expression level showed that MSMO1 gene expression was detected in 8 tissues. The expression level in liver was significantly higher than that in other 7 tissues (P0.01), and in spleen and pectoralis was significantly lower than that in lung and liver (P0.01). [conclusion] The structure and function of MSMO1 gene were studied.
【作者单位】： 临沂大学生命科学学院;
【基金】：山东省自然科学基金(ZR2017LC018,ZR2013CL012) 临沂大学校级大学生创新创业训练计划(201710452032) 国家自然科学基金(31372333)
【分类号】：S831
[Abstract]:[objective] to clone the MSMO1 (Methylsterol monooxygenase 1) gene sequence and analyze its expression pattern. [methods] the MSMO1 gene was cloned by RT-PCR technique and its protein structure was analyzed by bioinformatics. Quantitative PCR technique was used to detect the difference of the expression of MSMO1 gene among tissues. [results] the c DNA sequence of MSMO1 gene of broiler chicken was 1404 bp, encoding 296amino acid sequence residues of MSMO1 protein 145N 274, which existed in FA-hydroxylase domain and was hydrophilic protein. The phylogenetic tree analysis showed that the amino acid sequence of MSMO1 was closely related to the MSMO1 of Japanese quail and parrot, and the secondary structure was mainly 伪 -helix (41.89%). The results of tissue expression level showed that MSMO1 gene expression was detected in 8 tissues. The expression level in liver was significantly higher than that in other 7 tissues (P0.01), and in spleen and pectoralis was significantly lower than that in lung and liver (P0.01). [conclusion] The structure and function of MSMO1 gene were studied.
【作者单位】： 临沂大学生命科学学院;
【基金】：山东省自然科学基金(ZR2017LC018,ZR2013CL012) 临沂大学校级大学生创新创业训练计划(201710452032) 国家自然科学基金(31372333)
【分类号】：S831
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