丹芪祛瘀止痛颗粒对慢性萎缩性胃炎癌前病变大鼠PTEN、RUNX3基因甲基化的影响

发布时间：2018-09-03 10:02

【摘要】：目的:探讨丹芪祛瘀止痛颗粒对PLGC大鼠胃黏膜组织PTEN、RUNX3基因甲基化及PTEN、RUNX3基因mRNA表达的影响。方法:将80只大鼠按体质量随机分为空白组、模型组、维酶素组、丹芪祛瘀止痛颗粒组。除空白组外,其余各组均创建实验性大鼠PLGC模型。造模后,维酶素组和丹芪祛瘀止痛颗粒组给予相应药物灌胃,空白组和模型组以0.9%Nacl溶液灌胃,1次/d。灌胃12周后,观察各组胃组织病理学改变,MSP法检测PETN、Runx3基因甲基化及RT-PCR法检测PETN、Runx3 mRNA表达。结果:维酶素组、丹芪祛瘀止痛颗粒组均能改善胃黏膜组织炎症、萎缩、肠上皮化生、异型增生,其中以丹芪祛瘀止痛颗粒组最佳;造模后,模型组PTEN、RUNX3基因甲基化比率明显高于空白组(P0.01),药物干预后,维酶素组甲基化比率下调,与模型组比较,差异具有统计学意义(P0.05);丹芪祛瘀止痛颗粒组与模型组比较,差异具有显著统计学意义(P0.01),且丹芪祛瘀止痛颗粒组PTEN、RUNX3基因甲基化比率明显低于维酶素组(P0.01)。RT-PCR结果显示,模型组大鼠PTEN mRNA、RUNX3 mRNA较模型组显著下降(P0.01),药物干预后PTEN mRNA、RUNX3 mRNA显著上调,其中以丹芪祛瘀止痛颗粒组效果最佳,差异具有统计学意义(维酶素组P0.05,丹芪祛瘀止痛颗粒组P0.01)。结论:丹芪祛瘀止痛颗粒可明显改善PLGC大鼠胃黏膜组织萎缩、肠上皮化生及异型增生情况,其作用机制可能是通过降低基因甲基化比率,上调PTEN、RUNX3基因mRNA表达实现的。
[Abstract]:Aim: to investigate the effect of Danqi Quyu Zhitong granule (DQQZG) on PTEN,RUNX3 gene methylation and PTEN,RUNX3 gene mRNA expression in gastric mucosa of PLGC rats. Methods: 80 rats were randomly divided into blank group, model group, vascular enzyme group and Danqi Quyu Zhitong granule group. Experimental PLGC model was established in all groups except blank group. After the model was established, the rats in the vitamin group and Danqi Quyu Zhitong granule group were given the corresponding drugs intragastrically, and the blank group and the model group were perfused with 0.9%Nacl solution for one time per day. After 12 weeks of gastric perfusion, the histopathological changes of gastric tissues in each group were observed. The methylation of PETN,Runx3 gene and the expression of PETN,Runx3 mRNA were detected by RT-PCR method. Results: in the group of vitamin enzyme, Danqi Quyu Zhitong granule group could improve the inflammation, atrophy, intestinal metaplasia and dysplasia of gastric mucosa, among which Danqi Quyu Zhitong granule group was the best. The methylation rate of PTEN,RUNX3 gene in the model group was significantly higher than that in the blank group (P0.01). After drug intervention, the methylation rate of the vitamin group decreased significantly compared with the model group (P0.05). The difference was statistically significant (P0.01), and the methylation rate of PTEN,RUNX3 gene in Danqi Quyu Zhitong granule group was significantly lower than that in the vascular enzyme group (P0.01) .RT-PCR results showed that the PTEN mRNA,RUNX3 mRNA of model group was significantly lower than that of model group (P0.01), and PTEN mRNA,RUNX3 mRNA significantly increased after drug intervention. Among them, Danqi Quyu Zhitong granule group was the best, the difference was statistically significant (VT group P0.05, Danqi Quyu Zhitong granule group P0.01). Conclusion: Danqi Quyu Zhitong granule can obviously improve the atrophy of gastric mucosa, intestinal metaplasia and dysplasia in PLGC rats, and its mechanism may be by decreasing the ratio of gene methylation and up-regulating the expression of PTEN,RUNX3 gene mRNA.
【作者单位】：黑龙江省中医药科学院南岗分院消化科;
【基金】：国家中医药管理局科学技术专项项目(2016ZX05) 黑龙江省科技厅科技攻关项目(GC12C120) 黑龙江省博士后科研启动基金项目(LBH-Q13156)
【分类号】：R285.5

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