江汉油田区典型农田土壤烃类降解微生物功能基因bssA的遗传多样性研究
发布时间：2018-09-04 16:30
【摘要】：油田区土壤易受烃类物质影响并可能富集了特异的石油烃降解微生物类群。针对江汉油田区5个不同油井口附近的典型旱地农田土壤,采用石油烃(Petroleum hydrocarbons,PHs)中苯系物代谢的关键功能基因-bssA(苯甲基琥珀酸合成酶基因)作为分子标识物,通过克隆文库结合末端限制性片段长度多样性(Terminal-restriction fragment length polymorphism,T-RFLP)的方法,研究该油田区土壤含有bssA基因的烃类降解微生物群落结构,并探讨其环境驱动机制。结果表明,土壤中PAHs含量在0.21~2.01mg kg~(-1)之间,石油烃污染程度较低。T-RFLP的分析表明不同土壤样品中的bssA基因多样性差异明显,PAHs(Polycyclic Aromatic Hydrocarbons,多环芳烃)含量最高土壤中bssA基因多样性最高,其优势bssA基因类群与硫酸盐还原菌或地杆菌有较近的亲缘关系。冗余分析进一步表明,土壤硝态氮、有效磷、PAHs含量均是影响bssA基因多样性的重要因子。这些结果表明:江汉油田区典型农田土壤中含有bssA基因的主要类群为β-变形菌和δ-变形菌,并与地杆菌属(Geobacter)、索氏菌属(Thauera)和固氮菌属(Azoarcus)具有较近的系统发育亲缘关系。这些微生物可能通过硝酸盐、硫酸盐及铁还原代谢过程降解土壤PAHs。
[Abstract]:The soil in oilfield area is easy to be affected by hydrocarbon and may enrich the specific microbial community of petroleum hydrocarbon degradation. In this paper, the key functional gene of benzene metabolism in petroleum hydrocarbon (Petroleum hydrocarbons,PHs), -bssA (phenylmethyl succinate synthase gene), was used as molecular marker for typical dryland farmland soil near five different well orifices in Jianghan Oilfield. The community structure of hydrocarbon-degrading microorganism containing bssA gene in soil of the oilfield area was studied by means of cloning library combined with terminal restriction fragment length diversity (Terminal-restriction fragment length polymorphism,T-RFLP) and its environmental driving mechanism was discussed. The results showed that the content of PAHs in soil was between 0.21~2.01mg kg~ (1) and the degree of petroleum hydrocarbon pollution was lower. T-RFLP analysis showed that the diversity of bssA gene in different soil samples was significantly different from that of PAHs (Polycyclic Aromatic Hydrocarbons, polycyclic aromatic hydrocarbons (PAHs (Polycyclic Aromatic Hydrocarbons, polycyclic aromatic hydrocarbons). The dominant bssA gene group was closely related to sulfate reducing bacteria or Dibacillus. Redundancy analysis further showed that the contents of nitrate nitrogen and available phosphorus in soil were important factors affecting the diversity of bssA gene. The results showed that the main groups of bssA gene in typical farmland soil of Jianghan Oilfield were 尾 -Proteus and 未 -Proteus, and had close phylogenetic relationship with (Thauera) of (Geobacter), and (Azoarcus) of Azotobacter. These microorganisms may degrade soil PAHs. through nitrate, sulfate and iron reduction metabolism.
【作者单位】： 西南大学资源环境学院;
【基金】：国家自然科学基金项目(41371477) 中央高校专项基金项目(XDJK2014B047)资助~~
【分类号】：X172;X53
，
本文编号：2222723
[Abstract]:The soil in oilfield area is easy to be affected by hydrocarbon and may enrich the specific microbial community of petroleum hydrocarbon degradation. In this paper, the key functional gene of benzene metabolism in petroleum hydrocarbon (Petroleum hydrocarbons,PHs), -bssA (phenylmethyl succinate synthase gene), was used as molecular marker for typical dryland farmland soil near five different well orifices in Jianghan Oilfield. The community structure of hydrocarbon-degrading microorganism containing bssA gene in soil of the oilfield area was studied by means of cloning library combined with terminal restriction fragment length diversity (Terminal-restriction fragment length polymorphism,T-RFLP) and its environmental driving mechanism was discussed. The results showed that the content of PAHs in soil was between 0.21~2.01mg kg~ (1) and the degree of petroleum hydrocarbon pollution was lower. T-RFLP analysis showed that the diversity of bssA gene in different soil samples was significantly different from that of PAHs (Polycyclic Aromatic Hydrocarbons, polycyclic aromatic hydrocarbons (PAHs (Polycyclic Aromatic Hydrocarbons, polycyclic aromatic hydrocarbons). The dominant bssA gene group was closely related to sulfate reducing bacteria or Dibacillus. Redundancy analysis further showed that the contents of nitrate nitrogen and available phosphorus in soil were important factors affecting the diversity of bssA gene. The results showed that the main groups of bssA gene in typical farmland soil of Jianghan Oilfield were 尾 -Proteus and 未 -Proteus, and had close phylogenetic relationship with (Thauera) of (Geobacter), and (Azoarcus) of Azotobacter. These microorganisms may degrade soil PAHs. through nitrate, sulfate and iron reduction metabolism.
【作者单位】： 西南大学资源环境学院;
【基金】：国家自然科学基金项目(41371477) 中央高校专项基金项目(XDJK2014B047)资助~~
【分类号】：X172;X53
，
本文编号：2222723
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