风速对转基因棉花基因漂移的影响

发布时间：2018-09-05 05:48

【摘要】：在转基因作物全球商业化推广的20年间,公众对基因漂移生态安全问题的关注持续升温。本试验以转基因双价棉SGK321(Cry1Ac/Cp TI)为花粉供体,常规棉"石远321"和"中棉35"为花粉授体,借助PCR和蛋白检测技术,研究温室中3种风速(0.54、0.92和1.27 m·s-1)条件下的Cry1Ac基因漂移频率。结果表明:"石远321"作为花粉受体时,基因漂移频率受风速影响,低风速下最高,高风速下最低;"中棉35"作为花粉授体时,基因漂移频率整体较低,不受风速影响;基因漂移频率与所选择的花粉受体品种有关,"石远321"(转基因棉亲本)(10.63%)作为花粉受体时基因漂移频率高于"中棉35"(2.50%);转基因棉花基因漂移频率随着转基因作物种植区与非转基因作物之间距离的增加而下降。本试验为风介导的基因漂移的生态风险评估提供一定的理论参考。
[Abstract]:In the 20 years since the global commercialization of genetically modified crops, the public has been paying more attention to the ecological safety of gene drift. In this experiment, transgenic bivalent cotton SGK321 (Cry1Ac/Cp TI) was used as pollen donor, and conventional cotton "Shiyuan 321" and "Zhongmian35" as pollen donors. By means of PCR and protein detection techniques, the frequency of Cry1Ac gene drift in greenhouse under three wind speeds (0.54m / 0.92 and 1.27 m / s ~ (-1) was studied. The results showed that the gene drift frequency of "Shiyuan 321" as pollen receptor was affected by wind speed, the highest at low wind speed and the lowest at high wind speed, while the gene drift frequency of "Zhongmian35" as pollen donor was lower as a whole and not affected by wind speed. The gene drift frequency of "Shiyuan 321" (transgenic cotton parent) (10.63%) was higher than that of "Zhongmian35" (2.50%), and the gene drift frequency of transgenic cotton was higher than that of "Zhongmian35" (2.50%) when "Shiyuan 321" (transgenic cotton parent) was used as pollen receptor. The distance between the crops and non-transgenic crops increased and decreased. This study provides a theoretical reference for wind-mediated ecological risk assessment of gene drift.
【作者单位】：中国农业大学昆虫系;全国农业技术推广服务中心;北京出入境检验检疫局;柳州市农业技术推广中心;
【基金】：转基因棉花环境安全评价技术项目(2016ZX08011002)资助
【分类号】：S562

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