蝴蝶兰Rubisco活化酶基因PhRCAα的序列特征及在低温胁迫下的表达分析
发布时间：2018-09-05 09:50
【摘要】：为研究蝴蝶兰Rubisco活化酶基因(RCA)及其在低温胁迫下的响应机制,本研究利用RT-PCR及RACE技术从蝴蝶兰叶片中克隆了一个Rubisco活化酶基因PhRCAα的c DNA序列(登录号KU187968)该基因全长1 642 bp,编码473个氨基酸,包含P-loop_NTPase超家族结构域,预测其成熟蛋白的分子质量为46.16 k D,等电点为5.73,属于RCA基因的α亚基。实时荧光定量PCR分析显示,在13℃/8℃的昼夜温度条件下,蝴蝶兰PhRCAα基因的转录表达在处理3 d、6 d、9 d和15 d时明显下降,恢复正常温度条件后,该基因的表达升高;在4℃低温条件下,PhRCAα基因的转录表达在处理0.5 h、1 h和2 h内逐渐升高,而在处理4 h时其表达量下降,一直到低温处理48 h时,其表达量维持在较低水平。结果表明PhRCAα对驯化低温(13℃/8℃)和冷胁迫(4℃)具有不同的响应机制,PhRCAα对短期的冷胁迫有一定的防御作用。
[Abstract]:To study the mechanism of Rubisco activase gene (RCA) and its response to low temperature stress in Phalaenopsis. In this study, the c DNA sequence of Rubisco activase gene PhRCA 伪 (accession number KU187968) was cloned from the leaves of Phalaenopsis by RT-PCR and RACE techniques. The whole length of 1 642 bp, encodes 473 amino acids, including P-loop_NTPase superfamily domain. The molecular weight of the mature protein was 46.16 KD and the isoelectric point was 5.73, which belonged to the 伪 subunit of RCA gene. Real-time fluorescence quantitative PCR analysis showed that the transcriptional expression of PhRCA 伪 gene in Phalaenopsis was significantly decreased at 3 days, 6 days, 9 days and 15 days after treatment at 13 鈩，
本文编号：2223920
[Abstract]:To study the mechanism of Rubisco activase gene (RCA) and its response to low temperature stress in Phalaenopsis. In this study, the c DNA sequence of Rubisco activase gene PhRCA 伪 (accession number KU187968) was cloned from the leaves of Phalaenopsis by RT-PCR and RACE techniques. The whole length of 1 642 bp, encodes 473 amino acids, including P-loop_NTPase superfamily domain. The molecular weight of the mature protein was 46.16 KD and the isoelectric point was 5.73, which belonged to the 伪 subunit of RCA gene. Real-time fluorescence quantitative PCR analysis showed that the transcriptional expression of PhRCA 伪 gene in Phalaenopsis was significantly decreased at 3 days, 6 days, 9 days and 15 days after treatment at 13 鈩，
本文编号：2223920
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