茶树HD-Zip转录因子基因的克隆及其对非生物胁迫的响应分析

发布时间：2018-09-05 18:51

【摘要】：茶树作为一种叶用植物,具有重要的经济价值。非生物胁迫对茶树的生长发育过程和茶叶的生产影响很大。本研究采用RT-PCR方法从茶树‘龙井43’cDNA中克隆获得1个编码HD-Zip转录因子的基因CsHB1。序列分析显示,CsHB1基因cDNA长为1 380 bp,编码459个氨基酸,含有典型的START结构域。进化分析显示,茶树CsHB1属于HD-Zip家族转录因子的IV亚族。多序列对比发现,CsHB1与其他物种的HD-Zip类蛋白的氨基酸序列具有78.05%的相似性,如葡萄、烟草、芝麻等。对CsHB1转录因子理化性质、亲/疏水性、无序化分析显示,CsHB1转录因子是疏水性蛋白且显偏碱性,不存在无序化区域。空间结构分析显示,CsHB1有3个α-螺旋,多个β-折叠,且有START结构域。利用荧光定量PCR方法分析了CsHB1在高温(38°C)、低温(4°C)、PEG干旱(200 g·L~(-1))、NaCl(200 mmol·L~(-1))处理1、4、8、12 h的表达情况。结果表明,CsHB1基因在不同非生物胁迫处理下均能诱导表达,且表达差异性明显。
[Abstract]:As a kind of leaf plant, tea plant has important economic value. Abiotic stress has a great effect on the growth and development of tea plants and tea production. In this study, a gene encoding HD-Zip transcription factor, CsHB1., was cloned from tea plant 'Longjing 43'cDNA by RT-PCR method. Sequence analysis showed that the cDNA length of CsHB1 gene was 1 380 bp, encoding 459 amino acids, which contained a typical START domain. Phylogenetic analysis showed that tea CsHB1 belonged to the IV subfamily of transcription factors of HD-Zip family. The amino acid sequence of CsHB1 was 78.05% similar to that of other species, such as grape, tobacco, sesame and so on. The physicochemical properties, hydrophobicity and disordering of CsHB1 transcription factors showed that CsHB1 transcription factors were hydrophobic proteins and alkalescent, and there was no disorder region. The spatial structure analysis shows that CsHB1 has three 伪 -helix, many 尾 -folds and START domain. The expression of CsHB1 in high temperature (38 掳C), low temperature (4 掳C), drought (200g L ~ (-1) NaCl (200 mmol L ~ (-1) treatment for 812 h was analyzed by fluorescence quantitative PCR. The results showed that CsHB1 gene could be induced to express under different abiotic stress, and the difference of expression was significant.
【作者单位】：南京农业大学园艺学院茶叶科学研究所;
【基金】：国家自然科学基金(31570691)~~
【分类号】：Q943.2;S571.1

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