人输卵管糖蛋白-1基因甲基化状态与哈萨克族原发性高血压的相关性研究

发布时间：2018-09-09 12:00

【摘要】：目的:研究人输卵管糖蛋白-1(OVGP1)甲基化状态与哈萨克族原发性高血压的相关性。方法 :于2014年11月-2015年3月对新疆维吾尔自治区哈萨克族聚集区调查现场进行收集新疆哈萨克族原发性高血压组及正常对照组,提取外周血单个核细胞全基因组DNA。通过DNA甲基化芯片高通量筛选,筛选出相关位点再进行后续焦磷酸测序及荧光定量PCR进行验证分析。结果:高血压组与血压正常组组间得到差异甲基化位点总数目为75个,高甲基化位点43个,低甲基化位点32个。其中,发现OVGP1的甲基化水平在高血压组中呈低甲基化状态(P=0.04)。在焦磷酸测序验证后,位于OVGP1基因启动区的cg20823859位点的甲基化水平在高血压病例组中较正常对照组显示降低(P=1.88×10~(-5));然后再进行荧光定量PCR检测出OVGP1基因的表达水平在高血压病例组较正常对照组显示增高(P=3.92×10~(-7))。结论:OVGP1的低甲基化状态可能参与哈萨克族原发性高血压的发生发展。
[Abstract]:Objective: to study the correlation between the methylation of human tubal glycoprotein 1 (OVGP1) and Kazakh essential hypertension. Methods: from November 2014 to March 2015, we collected Xinjiang Kazakh essential hypertension group and normal control group, and extracted the whole genome DNA. of peripheral blood mononuclear cells from Xinjiang Uygur Autonomous region. By high throughput screening of DNA methylation chip, the related sites were screened for follow-up pyrosequencing and fluorescence quantitative PCR for verification and analysis. Results: the total number of methylation sites, hypermethylation sites and hypomethylation sites were 75, 43 and 32, respectively. The methylation level of OVGP1 was found to be hypomethylated in hypertensive group (P0. 04). After pyrosequencing, The methylation level of cg20823859 locus located in the promoter region of OVGP1 gene was significantly lower in hypertensive patients than that in normal controls (P < 1.88 脳 10 ~ (-5);). Then fluorescence quantitative PCR was used to detect the expression of OVGP1 gene in hypertensive patients (P _ (3.92) 脳 10 ~ (-7). Conclusion the hypomethylation of OVGP1 may be involved in the occurrence and development of essential hypertension in Kazak nationality.
【作者单位】：新疆石河子大学医学院;石河子大学医学院第一附属医院心内科;
【基金】：国家自然科学基金(编号:81460075)
【分类号】：R544.11

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