大蒜MADS-box基因AsPI的克隆及表达分析

发布时间：2018-09-11 06:33

【摘要】：B类MADS-box基因在调控显花植物的花瓣和雄蕊发育过程中发挥关键作用。为初步了解大蒜花发育的分子机制,以‘阿城紫皮’大蒜(Allium sativum)的花蕾为试材克隆了AsPI(Gen Bank登录号为KY272748)。AsPI的cDNA长939 bp,开放阅读框为615 bp,编码204个氨基酸。推导的氨基酸序列显示,AsPI编码的蛋白质包含高度保守的MADS结构域(1~57氨基酸)和保守的K结构(76~140氨基酸),AsPI蛋白具有PI亚家族特有的序列特征:MADS区特征丝氨酸残基、K区高度保守序列KHExL以及C 末端的PI基序。序列比对、功能域分析及系统发育分析结果表明,AsPI属于单子叶植物B功能基因PI亚家族。半定量和实时RT-PCR检测AsPI在大蒜不同组织器官中的表达模式,结果表明,在花蕾中高丰度表达,在花茎中表达量中等,在根、假茎、嫩叶、保护叶等营养器官中表达量极低甚至不表达。
[Abstract]:Class B MADS-box gene plays a key role in regulating the development of petals and stamens in flowering plants. In order to understand the molecular mechanism of garlic flower development, the cDNA length 939 bp, open reading frame of cDNA 939 bp, encoding 204 amino acids was cloned from the flower buds of garlic (Allium sativum) (AsPI (Gen Bank accession number KY272748). The deduced amino acid sequence shows that the protein encoded by Aspi contains highly conserved MADS domain (1 / 57 amino acid) and conserved K structure (76 / 140 amino acid). The ASPI protein has the specific sequence characteristic of the PI subfamily: the serine residue K of the Mads region. The region highly conserved sequence KHExL and the PI motif at the end of C ~ +. Sequence alignment, functional domain analysis and phylogenetic analysis showed that AsPI belonged to the PI subfamily of B functional genes in monocotyledonous plants. Semi-quantitative and real-time RT-PCR were used to detect the expression pattern of AsPI in different tissues and organs of garlic. The results showed that the expression of AsPI was high in flower bud, moderate in flower stem, and in root, pseudostem and tender leaf. Protective leaves and other vegetative organs in the expression of very low or non-expression.
【作者单位】：东北林业大学生命科学学院;杭州和壹基因科技有限公司;
【基金】：黑龙江省应用技术研究与开发计划重大项目(GA15B103-7)
【分类号】：S633.4

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