胡桃楸成花相关LFY同源基因的克隆和序列分析

发布时间：2018-09-11 08:05

【摘要】：通过对胡桃楸成花相关LFY基因进行研究,对缩短胡桃楸童期,促进其提早开花结实有着极为重要的意义。采取PCR扩增技术,在NCBI数据库中进行搜索,查找到与目的基因有关联的同源序列;并根据这些有关联的序列来进行引物设计,扩增目的基因全长。克隆LFY基因cDNA序列全长为1 297 bp,其中CDS序列长1 158 bp,编码385个氨基酸。NCBI数据库Blast比对表明胡桃楸LFY同源基因与其他物种的LFY同源基因同源性均在80%以上。将胡桃楸LFY同源基因命名为JmLFY(GenBank登录号:KX364241)。通过BioEdit软件和ProtParam ExPASy在线软件分析胡桃楸JmLFY蛋白质分子式为C_(1878)H_(2962)N_(566)O_(578)S_(13),分子量为43 134.4 Da;理论等电点pI值为6.78;不稳定指数为39.92,属于不稳定蛋白质。该研究为进一步探索LFY基因对胡桃楸成花的影响提供依据。
[Abstract]:The study of floral related LFY gene of Juglans mandshurica is of great significance in shortening the childhood stage of Juglans mandshurica and promoting its early flowering and fruiting. The PCR amplification technique was used to search the NCBI database to find the homologous sequences associated with the target gene, and the primers were designed to amplify the full length of the target gene. The length of cDNA sequence of LFY gene was 1 297 bp, and the length of CDS sequence was 1 158 bp, encoding 385 amino acids. Blast alignment of NCBI database showed that LFY homology gene of Juglans mandshurica was more than 80% homology with that of other species. The homologous gene of Juglans mandshurica LFY was named JmLFY (GenBank accession number: KX364241). The molecular formula of JmLFY protein of Juglans mandshurica was C _ (1878) H _ (2962) N _ (566) O _ (578) S _ (13), the molecular weight was 43 134.4 Da;, the pI value of isoelectric point was 6.78, and the instability index was 39.92, which was an unstable protein. This study provides the basis for further exploring the effect of LFY gene on flower formation of Juglans mandshurica.
【作者单位】：沈阳农业大学林学院;辽宁省固沙造林研究所;东北林业大学林学院林木遗传育种国家重点实验室;
【基金】：辽宁省自然科学基金项目(20170540801)资助
【分类号】：Q943.2;S792.132

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