叔丁基对羟基茴香醚对雨生红球藻虾青素积累和psy基因表达量的影响

发布时间：2018-09-13 09:41

【摘要】：研究胁迫培养条件下,在对数生长期的雨生红球藻Haematococcus pluvialis LUGU藻液中添加不同质量浓度叔丁基对羟基茴香醚(butyl hydroxyanisole,BHA)对其影响,并分别对细胞生物量、虾青素积累量以及八番茄红素合成酶psy基因表达量进行了测定。结果表明,BHA质量浓度为2 mg/L时,虾青素积累量显著提高,最大含量可达29.03 mg/g,是对照组(14.30 mg/g)的2.03倍。而在相同的BHA诱导条件下,初始藻细胞的不同生长状态对细胞生长及虾青素合成也有很大影响:处于对数后期(14 d)的细胞其最大虾青素含量可达29.3 mg/g,分别是对数前期、中期和稳定期的2.93、1.01倍和1.73倍。实时荧光定量聚合酶链式反应分析显示,虾青素合成关键基因psy的表达受BHA的影响,当对数后期(14 d)添加2 mg/L BHA时,psy基因相对表达水平为对照9.2倍。结果表明,适当质量浓度BHA不仅能够显著提高虾青素合成关键酶基因psy的表达水平,并且明显促进了微藻细胞内虾青素积累量,为虾青素产业化提供了一种有效的策略。
[Abstract]:The effects of different concentrations of tert-Ding Ji on hydroxy anisole (butyl hydroxyanisole,BHA) and cell biomass in logarithmic growth phase of Haematococcus pluvialis LUGU algae were studied. Astaxanthin accumulation and psy gene expression of lycopene synthase were determined. The results showed that the content of astaxanthin increased significantly when the concentration of BHA was 2 mg/L, and the maximum content of astaxanthin was 2.03 times of that of the control group (14.30 mg/g), and the maximum content of astaxanthin was 29.03 mg/g,. Under the same BHA induction condition, different growth states of initial algal cells also had a great effect on cell growth and astaxanthin synthesis: the maximum astaxanthin content of the cells at the late logarithmic stage (14 days) was 29.3 mg/g, respectively. 1.01 times and 1.73 times of the medium and stable period. Real-time fluorescence quantitative polymerase chain reaction analysis showed that the expression of astaxanthin synthesis key gene psy was affected by BHA, and the relative expression level of astaxanthin gene was 9.2 times higher than that of control when 2 mg/L BHA was added at the end of logarithmic period (14 days). The results showed that the appropriate concentration of BHA could not only significantly increase the expression level of astaxanthin synthesis key enzyme gene psy, but also promote the accumulation of astaxanthin in microalgae cells, which provided an effective strategy for astaxanthin industrialization.
【作者单位】：昆明理工大学生命科学与技术学院;
【基金】：国家自然科学基金地区科学基金项目(21266013)
【分类号】：Q943.2;Q946

【相似文献】