水产品批发市场中抗生素抗性基因的污染分析

发布时间：2018-09-14 18:52

【摘要】：为了探究待售水产品中抗生素抗性基因(Antibiotic resistance genes,ARGs)在水产品批发市场中的扩散污染情况,选取上海市3个大型水产品批发市场作为研究对象,采用PCR的方法,对市场内部和市场附近的排污沟表层淤泥中5大类(4种磺胺类、8种四环素类、6种β-内酰胺类、5种氯霉素类、4种链霉素类)共27种ARGs进行了检测,并利用PCR-DGGE技术分析了细菌种群多样性。结果显示共有17种ARGs在批发市场淤泥中被检出,其中磺胺类、四环素类和链霉素类ARGs的检出率较高且检出种类较多,表明水产品批发市场含有种类丰富的ARGs,水产品以磺胺类、四环素类和链霉素类ARGs污染为主。对市场外围的检测显示市场外围区域ARGs的种类分布略低于市场内部,表明水产品批发市场中ARGs的存在会通过污水排放途径扩散到周边环境。DGGE图谱的分析结果显示,3个批发市场污泥样品细菌种类差异性较大,表明环境中ARGs的种类分布与细菌的种类并无直接关联。本研究表明水产品携带的ARGs会扩散到水产品批发市场中,导致水产品批发市场成为ARGs的重要储存库,以此造成的污染会进一步传播到周边环境,此外,这些种类丰富的ARGs重新扩散到待售水产品中的风险很大,需引起人们足够的重视。
[Abstract]:In order to investigate the diffusion pollution of antibiotic resistance gene (Antibiotic resistance genes,ARGs) in aquatic products in wholesale market of aquatic products, three large-scale aquatic products wholesale markets in Shanghai were selected as the research object, and the method of PCR was used. A total of 27 kinds of ARGs were detected in the surface silt of sewer ditch in the market and near the market, including 4 kinds of sulfamides, 8 kinds of tetracycline, 6 kinds of 尾 -lactam, 5 kinds of chloramphenicol and 4 kinds of streptomycin. The diversity of bacterial population was analyzed by PCR-DGGE technique. The results showed that 17 kinds of ARGs were detected in the mud of wholesale market. The detection rate of sulfonamides, tetracyclines and streptomycin ARGs was high and there were many kinds of ARGs, indicating that there were many kinds of ARGs, aquatic products in the wholesale market of aquatic products. Tetracycline and streptomycin are mainly polluted by ARGs. Tests on the periphery of the market show that the category distribution of ARGs in the periphery of the market is slightly lower than that within the market. The results showed that the existence of ARGs in the wholesale market of aquatic products would spread to the surrounding environment through the way of sewage discharge. The results showed that the bacterial species of sludge samples in the three wholesale markets were quite different. The results showed that there was no direct relationship between the species distribution of ARGs and the species of bacteria in the environment. This study shows that the ARGs carried by aquatic products will spread to the wholesale market of aquatic products, leading to the wholesale market of aquatic products become an important repository of ARGs, and the pollution caused by this will be further spread to the surrounding environment. The risk of rediffusion of these rich ARGs into aquatic products for sale is high enough to attract enough attention.
【作者单位】：上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心;农业部水产品贮藏保鲜质量安全风险评估实验室(上海);
【基金】：国家自然科学基金项目(31271870) 上海市科委计划项目(14320502100) 上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号) 上海水产品加工及贮藏工程技术研究中心项目(11DZ2280300)
【分类号】：S948

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