水稻白化转绿基因Gra3的精细定位及遗传分析

发布时间：2018-09-15 06:37

【摘要】：本研究鉴定到一份水稻苗期白化转绿突变体,该突变体在幼苗的2叶到4叶期会出现叶片边缘白化,4叶期后转绿,对突变体和野生型农艺性状比较研究表明,前期白化不会对植株生长发育及产量等相关性状产生影响;同时,对两者叶绿体超微结构对比分析发现,突变体叶绿体发育在白化期受到一定程度的抑制。通过构建F2分离群体,调查了部分F2单株表型,结果表明白化型和野生型单株符合1:3的孟德尔单基因分离比,遂将该基因命名为Gra3;利用分离群体分析法(bulked segregant analysis,BSA)和隐性群体分析法(recessive-class analysis,RCA)最终将Gra3定位于RM14436和RM14450之间,对应日本晴基因组片段125 kb。该性状可作为一个形态学标记应用于水稻生产。
[Abstract]:In this study, a rice albino to green mutant was identified. The mutant could turn green at the stage of 2 to 4 leaves. The comparison of agronomic characters between the mutant and the wild type showed that the leaf edge of the mutant was albino at the stage of albinism and that of the second leaf of the seedling was turned green after the stage of the fourth leaf. Early albinism had no effect on plant growth and yield, and the ultrastructure of chloroplast was inhibited to some extent during albinism. The phenotypes of some F2 plants were investigated by constructing a F2 segregation population. The results showed that albino and wild type plants were in accordance with the 1:3 Mendelian segregation ratio. The gene was named Gra3; and recessive population analysis (recessive-class analysis,RCA) and (bulked segregant analysis,BSA). Finally, Gra3 was located between RM14436 and RM14450, corresponding to the Japanese genome 125kb.. This trait can be used as a morphological marker in rice production.
【作者单位】：湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室;江西省超级水稻研究发展中心/水稻国家工程实验室;湖北省农业科学院科技处;
【基金】：湖北省自然科学基金(2015CFC893) 国家973计划课题(2013CBA01405) 湖北省农业科技创新中心资助项目(2007-620-001-003)共同资助
【分类号】：S511

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