白术多糖对断奶仔猪白细胞介素-1β基因表达的影响

发布时间：2018-09-15 19:34

【摘要】：本试验选用44日龄、平均体重8.94 kg的DLY三元杂交断奶仔猪60头,随机分为5个处理组,每个处理3个重复,每个重复4头猪。第一组为对照组,饲喂基础日粮;第二、第三、第四和第五组为试验组,分别在基础日粮中添加抗生素(杆菌肽锌+硫酸粘杆菌素)及0.05%、0.10%、0.15%的白术多糖(PAM),正式期为30 d。30 d后颈静脉采取血液样品,并应用SYBR Green I荧光染料实时定量PCR方法进行白细胞介素-1β(IL-1β)基因mRNA的定量,研究白术多糖对断奶仔猪免疫功能的影响,为白术多糖的深入研究提供科学依据。结果表明:IL-β基因的相对表达量,各处理组间差异不显著(P0.05),但白术多糖处理组均比对照组和抗生素组高,其中以0.1%PAM组最高,抗生素组、0.05%PAM组次之,对照组和0.15%PAM处理组的最低。0.1%PAM处理组与对照组和抗生素组相比,IL-β基因相对表达量分别提高34.21%和27.5%;0.1%PAM处理组与0.05%PAM处理组和0.15%PAM处理组相比,IL-β基因相对表达量分别提高27.5%和34.21%。0.05%PAM处理组与对照组和抗生素组相比,IL-β基因相对表达量分别提高5.2%和0%;0.15%PAM处理组与对照组相比,IL-β基因相对表达量提高0%;0.15%PAM处理组与抗生素组相比,IL-β基因相对表达量降低5%。
[Abstract]:In this experiment, 60 DLY weaned piglets with average weight of 8.94 kg and 44 days old were randomly divided into 5 treatment groups, 3 replicates per treatment and 4 piglets per replica. The first group was the control group, fed the basic diet, and the second, third, fourth and fifth groups were the experimental groups. Blood samples were taken from jugular vein after 30 days after adding antibiotics (Bacteriopin zinc sulfate) and 0.05% Atractylodes macrocephala polysaccharides (PAM),) in basal diet for 30 d, respectively, and 0.15% of Atractylodes macrocephala polysaccharides (PAM),) were added to the basal diet for 30 days. The effect of Atractylodes macrocephala polysaccharides on the immune function of weaned piglets was studied by using real-time quantitative PCR method of SYBR Green I fluorescent dye to quantify the mRNA of IL-1 尾 gene, which provides a scientific basis for the further study of Atractylodes macrocephala polysaccharides in weaning piglets. The results showed that there was no significant difference in the relative expression of IL-尾 gene among different treatment groups (P0.05), but the Atractylodes macrocephala polysaccharide treatment group was higher than the control group and the antibiotic group, the highest in the 0.1%PAM group, followed by 0.05 PAM group in the antibiotic group. The relative expression of IL- 尾 gene increased by 34.21% in the control group and 27.50.1% in the 0.15%PAM treatment group compared with the control group and the antibiotic treatment group. The relative expression of the IL- 尾 gene in the PAM-treated group was increased by 27.5% compared with the 0.05%PAM treatment group and the 0.15%PAM treatment group, and the relative expression of IL- 尾 gene in the PAM-treated group was increased by 27.5% compared with the 0.05%PAM treatment group and the 0.15%PAM treatment group. Compared with the control group and the antibiotic group, the relative expression of IL- 尾 gene in the 34.21%.0.05%PAM treatment group was increased by 5.2%, and the relative expression level of the IL- 尾 gene in the 0 ~ (15) PAM group was increased by 0% compared with the control group. The relative expression of IL- 尾 gene in the 34.21%.0.05%PAM treatment group was decreased by 5% compared with the antibiotic group.
【作者单位】：芒市科技局科协;云南农业大学动物科学学院省动物营养与饲料科学重点实验室;潍坊六和饲料有限公司临朐分公司;芒市畜牧局;德宏州畜牧局;德宏州科技局科协;
【基金】：云南省运用基础研究面上基金项目(2007C0056M)
【分类号】：S828.5

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