蝴蝶兰C类花器官发育基因PhAG1a的克隆及表达分析
发布时间：2018-10-04 20:34
【摘要】：为研究MADS-box基因在花器官发育中的功能,进一步理解兰科植物花器官发育的分子调控机理。利用RT-PCR和RACE技术从蝴蝶兰中克隆了一个MADS-box基因PhAG1a(GenBank登录号为KY399811),并利用实时荧光定量方法分析其表达特性。序列分析表明,该基因的cDNA全长为1 107 bp,含完整的开放阅读框,可编码248个氨基酸,属于C功能AGAMOUSE家族基因,与蝴蝶兰属的PhalAG1和PeMADS1基因关系最近;表达模式分析表明,PhAG1a基因在营养器官中不表达,只在蕊柱和子房中表达。在5类突变体花器官中,PhAG1a基因在退化雄蕊变异为侧瓣、侧瓣退化与蕊柱合生和侧萼片变异为唇瓣等3类突变体花器官中,PhAG1a基因的表达没有变化,在侧瓣变异为唇瓣和侧瓣顶端长出花药的突变体花器官中,PhAG1a基因在蕊柱中的表达水平显著降低。推测该基因在决定蝴蝶兰合蕊柱的发育中起重要的调控作用。
[Abstract]:In order to study the function of MADS-box gene in floral organ development, the molecular regulation mechanism of floral organ development in orchid plants was further understood. A MADS-box gene PhAG1a (GenBank accession number KY399811) was cloned from Phalaenopsis by RT-PCR and RACE techniques, and its expression characteristics were analyzed by real-time fluorescence quantitative method. Sequence analysis showed that the total length of cDNA was 1 107 bp, which contained a complete open reading frame, encoding 248 amino acids, belonging to the C-functional AGAMOUSE family, which had the closest relationship with the PhalAG1 and PeMADS1 genes of Phalaenopsis. The expression pattern analysis showed that the PhAG1a gene was not expressed in vegetative organs, but only in stamen and ovary. In floral organs of five mutants, the expression of PhAG1a gene was not changed in floral organs of three types of mutants, such as staminodes, lateral petal and stamen column, and lateral sepals, and so on, and the expression of PhAG1a gene was not changed in floral organs of the five mutants, and there was no change in the expression of PhAG1a gene in floral organs. The expression level of PhAG1a gene in the floral organs of the mutant which mutated to lip and the tip of the lateral valve was significantly decreased in the stamen column. It is speculated that this gene plays an important role in determining the development of syphalopsis in Phalaenopsis.
【作者单位】： 郑州师范学院生物工程研究所;郑州师范学院生命科学学院;
【基金】：河南省科技攻关项目(No.162102110073)
【分类号】：Q943.2;S682.31
，
本文编号：2251789
[Abstract]:In order to study the function of MADS-box gene in floral organ development, the molecular regulation mechanism of floral organ development in orchid plants was further understood. A MADS-box gene PhAG1a (GenBank accession number KY399811) was cloned from Phalaenopsis by RT-PCR and RACE techniques, and its expression characteristics were analyzed by real-time fluorescence quantitative method. Sequence analysis showed that the total length of cDNA was 1 107 bp, which contained a complete open reading frame, encoding 248 amino acids, belonging to the C-functional AGAMOUSE family, which had the closest relationship with the PhalAG1 and PeMADS1 genes of Phalaenopsis. The expression pattern analysis showed that the PhAG1a gene was not expressed in vegetative organs, but only in stamen and ovary. In floral organs of five mutants, the expression of PhAG1a gene was not changed in floral organs of three types of mutants, such as staminodes, lateral petal and stamen column, and lateral sepals, and so on, and the expression of PhAG1a gene was not changed in floral organs of the five mutants, and there was no change in the expression of PhAG1a gene in floral organs. The expression level of PhAG1a gene in the floral organs of the mutant which mutated to lip and the tip of the lateral valve was significantly decreased in the stamen column. It is speculated that this gene plays an important role in determining the development of syphalopsis in Phalaenopsis.
【作者单位】： 郑州师范学院生物工程研究所;郑州师范学院生命科学学院;
【基金】：河南省科技攻关项目(No.162102110073)
【分类号】：Q943.2;S682.31
，
本文编号：2251789
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