PD-L1基因多态性与肝癌易感性以及预后之间的关系

发布时间：2018-10-05 21:03

【摘要】：目的研究PD-L1基因多态性与肝癌易感性以及患者预后之间的关系。方法收集我院123例原发性肝癌患者和141例健康体检者的外周血淋巴细胞,应用直接测序法和PCR-SSCP检测PD-L1基因rs4143185,rs2890658和rs17718883三个位点的单核苷酸多态性。结果 rs2890658位点的AC基因型及rs17718883的CG基因型可以显著降低肝癌的发病风险(OR=0.43,P=0.035;OR=0.01,P0.001);而rs4143185的GG基因型可能增加肝癌的发病风险(OR=2.80,P=0.057)。rs2890658位点AC+CC和AA的中位生存时间分别为20个月和17个月(P=0.042);rs4143185位点的GC+GG和CC中位生存时间分别为21个月和15个月,差异有统计学意义(P0.001);COX回归分析显示rs4143185位点的突变基因型都可以获得好的预后,差异有统计学意义(HR1;且P0.05)。结论 PD-L1基因rs2890658和rs17718883两个位点的SNP与肝癌的易感性有关,而rs2890658和rs4143185位点的SNP可能与肝癌的预后有关。
[Abstract]:Objective to study the relationship between PD-L1 gene polymorphism and susceptibility to liver cancer and prognosis. Methods Peripheral blood lymphocytes were collected from 123 patients with primary liver cancer and 141 healthy controls. The single nucleotide polymorphisms at the rs4143185,rs2890658 and rs17718883 loci of PD-L1 gene were detected by direct sequencing and PCR-SSCP. Results the AC genotype of rs2890658 locus and CG genotype of rs17718883 could significantly reduce the risk of HCC (OR=0.43,P=0.035;OR=0.01,P0.001), while the GG genotype of rs4143185 could increase the risk of HCC (OR=2.80,P=0.057. Rs2890658) the median survival time of AC CC and AA were 20 months, respectively. The median survival time of GC GG and CC at rs4143185 was 21 months and 15 months, respectively. The difference was statistically significant (P0.001) Cox regression analysis showed that the mutation genotype of rs4143185 locus could obtain good prognosis (HR1; and P0.05). Conclusion SNP at rs2890658 and rs17718883 sites of PD-L1 gene is associated with susceptibility to HCC, while SNP at rs2890658 and rs4143185 loci may be related to prognosis of HCC.
【作者单位】：第三军医大学大坪医院野战外科研究所肿瘤中心;
【分类号】：R735.7

【相似文献】