张杂谷提取物的抗氧化研究及蔗糖合成酶基因家族的生物信息学分析

发布时间：2018-10-07 21:18

【摘要】：谷子(Setaria italica)是我国的传统粮食作物之一。其种植面积广泛,具有较高的营养价值和抗氧化能力。蔗糖合成酶是蔗糖代谢的关键酶,能够影响植物体内抗氧化物质的合成及抗氧化能力。因以,本课题共分为两个部分来研究,第一部分是谷子提取物的抗氧化作用研究,第二部分是谷子蔗糖合成酶基因家族生物信息学研究。本试验以5个不同的谷子品种为研究对象,通过采用分光光度法和高效液相法对谷子的去皮后获得的谷子提取物中总酚、黄酮、α-生育酚和多糖的含量和体外抗氧化的差异进行研究。结果显示不同品种多酚、黄酮、α-生育酚和多糖含量变化范围分别为79.31~36.99mg·100g-1、16.21~38.51 mg·100g-1、0.712~1.531 mg·100g-1和3.35~15.93mg·g-1。醇提物抗DPPH、OH-自由基清除率和总抗氧化能力的IC50值范围分别为0.09~0.24mg·mL-1、0.41~0.74 mg·mL-1和0.98~2.35mg·mL-1。水提物抗DPPH、OH-自由基清除率和总抗氧化能力与醇提物相比较弱。相关性分析结果表明谷子醇提取物的DPPH清除率与多酚、α-生育酚和黄酮的相关较大;对OH-的清除率与多酚、黄酮的相关性较大;而对于总抗氧化能力与α-生育酚相关性较大。通过分析发现不同谷子品种抗氧化物质的含量和抗氧化特性有一定差异,谷子抗氧化能力与品种间抗氧化成分含量的高低间没有明显的量效关系,醇提物抗氧化能力强于水提物,5个谷子品种中张杂谷3号有较强的抗氧化活性。以谷子基因组数据库为平台,利用生物信息学方法对谷子SuS基因(Si SuS)家族进行挖掘和分析。结果表明,谷子的SiSuS基因家族包括9个基因,它们不均匀的分布在5条染色体上。SiSuS特性分析表明该家族氨基酸序列长度为809~1088aa,外显子数目为10~16,大多数蛋白质属弱酸性。MEME软件分析显示,谷子SuS的氨基酸序列具有9个保守基序;进化树分析表明谷子、水稻和高粱SuS基因具有一定的同源性。研究结果为进一步研究谷子抗氧化机制提供了理论依据。
[Abstract]:Millet (Setaria italica) is one of the traditional grain crops in China. Its planting area is extensive and has high nutritional value and anti-oxidation ability. Sucrose synthase is a key enzyme in sucrose metabolism, which can affect the synthesis and antioxidant capacity of antioxidants in plants. As a result, this subject is divided into two parts: the first part is about the antioxidant effect of millet extract, the second part is the bioinformatics study of sucrose synthase gene family in millet. In this experiment, five different millet varieties were used as research objects. The total phenol in the extract of millet was obtained by spectrophotometry and high performance liquid phase method. The contents of flavonoids, 伪-tocopherols and polysaccharides and the difference of antioxidant activity in vitro were studied. The results showed that the contents of polyphenols, flavonoids, 伪 -tocopherols and polysaccharides in different varieties varied from 79.31~36.99mg 100g-1 to 16.21C 38.51 mg 100g-1 and 3.35~15.93mg g-1. 531 mg 100g-1, respectively. The range of IC50 value of alcohol extract against DPPH,OH- radical scavenging rate and total antioxidant capacity is 0.09~0.24mg mL-1,0.41~0.74 mg mL-1 and 0.98~2.35mg mL-1., respectively. The scavenging rate and total antioxidant ability of water extract against DPPH,OH- free radical were weaker than that of alcohol extract. The results of correlation analysis showed that the DPPH clearance rate of millet alcohol extract was significantly correlated with polyphenols, 伪 -tocopherol and flavonoids, the clearance of OH- with polyphenols and flavonoids, and the total antioxidant capacity with 伪 -tocopherol. It was found that there were some differences in the contents and characteristics of antioxidants in different millet varieties, and there was no significant dose-effect relationship between the antioxidant capacity of millet and the content of antioxidant components in different millet varieties. The antioxidant activity of alcohol extract was stronger than that of water extract, and Zhangzagu 3 had strong antioxidant activity in 5 millet varieties. On the platform of millet genome database, the SuS gene (Si SuS) family of millet was mined and analyzed by bioinformatics. The results showed that the SiSuS gene family of millet consists of nine genes. The amino acid sequence length of the family was 809 ~ 1088aa and the number of exons was 10 ~ (16). The amino acid sequence of millet SuS had 9 conserved motifs. Phylogenetic tree analysis showed that the SuS gene of millet, rice and sorghum had some homology. The results provide theoretical basis for further study on antioxidation mechanism of millet.
【学位授予单位】：河北北方学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：Q946

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