TRAIL基因修饰的树突状细胞诱导黑色素瘤细胞凋亡的实验研究

发布时间：2018-10-07 20:09

【摘要】：为研究TRAIL基因修饰的树突状细胞诱导黑色素瘤细胞凋亡的作用,研究用携带TRAIL基因的重组腺病毒转染小鼠来源的树突状细胞(dendritic cell,DC),采用流式细胞术(flow cytometry,FCM)检测DC TRAIL蛋白的表达水平;将DC-TRAIL与B16黑色素瘤细胞混合培养,显微镜观察细胞生长情况,FCM检测B16细胞的凋亡情况;构建小鼠黑色素瘤模型,分别将DC-TRAIL、DC和PBS皮下注射于肿瘤接种部位,观察小鼠的瘤体生长情况,测量瘤体的体积。结果显示:用Ad-TRAIL转染DC后DC可高表达TRAIL。DC-TRAIL组与DC组相比可明显诱导肿瘤细胞凋亡。用DC-TRAIL、DC和PBS处理黑色素瘤移植小鼠2周后,发现DC-TRAIL组小鼠平均肿瘤体积为(0.33±0.10)cm~3,DC组小鼠平均肿瘤体积为(1.32±0.29)cm~3,PBS组小鼠平均肿瘤体积为(3.01±0.52)cm~3。与DC组和PBS组相比,DC-TRAIL组可明显抑制肿瘤生长。
[Abstract]:In order to study the effect of TRAIL gene modified dendritic cells on the apoptosis of melanoma cells, the recombinant adenovirus carrying TRAIL gene was used to transfect dendritic cells (dendritic cell,DC) from mice. Flow cytometry (flow cytometry,FCM) was used to detect the expression of DC TRAIL protein. The DC-TRAIL and B16 melanoma cells were mixed cultured, the cell growth was observed by microscope, the apoptosis of B16 cells was detected by FCM, the mouse melanoma model was established, and the DC-TRAIL,DC and PBS were injected subcutaneously into the tumor inoculation site, respectively. The growth of mouse tumor was observed and the volume of tumor was measured. The results showed that the high expression of DC in DC transfected with Ad-TRAIL could induce apoptosis of tumor cells in TRAIL.DC-TRAIL group compared with DC group. After treated with DC-TRAIL,DC and PBS for 2 weeks, the average tumor volume of mice in DC-TRAIL group was (0.33 卤0.10) cm~3,DC and that in cm~3,PBS group was (1.32 卤0.29). The mean tumor volume in cm~3,PBS group was (3.01 卤0.52) cm~3.. Compared with DC group and PBS group, DC-trail group could significantly inhibit tumor growth.
【作者单位】：上海交通大学医学院附属第九人民医院;
【基金】：上海市宝山区科委资助项目(13-E3)
【分类号】：R730.51

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