甘蔗线条花叶病毒HC-Pro基因的分子变异分析

发布时间：2018-10-07 19:53

【摘要】：为了解析甘蔗线条花叶病毒Sugarcane streak mosaic virus(SCSMV)不同分离物HC-Pro基因的分子变异规律,本研究利用RT-PCR法扩增获得SCSMV HC-Pro基因的序列,通过生物信息学分析,分别从重组、系统发生、选择压力等方面研究SCSMV HC-Pro基因的分子变异特征。共测定了44条SCSMV HC-Pro基因序列,相似性最低值为70%;HC-Pro基因重组频率较低,仅发现3个重组位点,其中一个系首次报道;与先前报道相比,部分新测定云南蔗区的SCSMV分离物在HC-Pro基因上形成一个新组-第Ⅲ组;HC-Pro基因处于很强的负选择压力作用,未发现正向选择作用位点。本研究结果进一步证明SCSMV HC-Pro基因具有高度的遗传多样性。
[Abstract]:In order to analyze the rule of molecular variation of HC-Pro gene of sugarcane line mosaic virus (Sugarcane streak mosaic virus (SCSMV), the sequence of SCSMV HC-Pro gene was amplified by RT-PCR method. The sequence of SCSMV HC-Pro gene was sequenced from recombination and phylogeny by bioinformatics analysis. The molecular variation characteristics of SCSMV HC-Pro gene were studied in the aspect of selection pressure. A total of 44 SCSMV HC-Pro gene sequences were sequenced. The lowest similarity was that the recombination frequency of HC-Pro gene was low, and only three recombination sites were found, one of which was reported for the first time. Part of the newly determined SCSMV isolates from sugarcane region of Yunnan Province formed a new group on the HC-Pro gene. The HC-Pro gene of group 鈪，

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