白菜叶形发育相关基因BrLOM2的克隆与表达分析

发布时间：2018-10-08 08:34

【摘要】：为了揭示白菜(Brassica rapa ssp.chinensis)BrLOM2基因在叶发育中的调控机制,以白菜圆叶、裂叶近等基因系为材料,同源克隆得到BrLOM2基因,分析其时空表达特性及对激素的响应。结果显示,BrLOM2的ORF全长为549 bp,编码182个氨基酸,含有GRAS家族保守结构域,定位于细胞核,两种材料中的氨基酸序列在保守结构域存在两处突变。进化分析表明,BrLOM2属于十字花科LOM2分支,与大白菜(XP_009138823.1)亲缘关系最近。RT-PCR结果显示,BrLOM2在圆叶白菜中的表达量高于同期裂叶白菜,两种材料茎尖、第1片叶中表达量最高;裂叶中的表达量与叶缘裂刻数呈现同步增加的趋势,6-BA和GA_3分别抑制和促进叶片中的表达,说明BrLOM2低表达可能是裂叶表型产生的主要原因。
[Abstract]:In order to elucidate the regulatory mechanism of (Brassica rapa ssp.chinensis) BrLOM2 gene in leaf development, BrLOM2 gene was cloned homologous from Chinese cabbage round leaf and lobed leaf lines, and its spatio-temporal expression characteristics and response to hormones were analyzed. The results showed that the total length of ORF of BrLOM2 was bp, encoding 182 amino acids, which contained the conserved domain of GRAS family and located in the nucleus. The amino acid sequence of the two materials had two mutations in the conserved domain. Phylogenetic analysis showed that BrLOM2 belonged to the LOM2 branch of Cruciferae. The results of RT-PCR showed that the expression of BrLOM2 in Chinese cabbage was higher than that in Chinese cabbage at the same time. The highest expression level was found in the first leaf of the two materials. The expression of 6-BA and GA_3 increased synchronously with the number of cleavage in lobed leaves, indicating that the low expression of BrLOM2 might be the main cause of the phenotypic formation of lobed leaves, and 6-BA and GA_3 could inhibit and promote the expression in leaves, respectively.
【作者单位】：西北农林科技大学园艺学院农业部西北地区园艺作物生物学与种质资源创新重点实验室;
【基金】：国家自然科学基金项目(31372062,31272164) 陕西省农业科技创新与攻关项目(2015NY103)
【分类号】：S634.3

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