石蒜LrCMO基因的克隆及表达分析
发布时间：2018-10-13 08:47
【摘要】：以石蒜(Lycoris radiata)为试材,采用同源克隆和RACE的试验方法,克隆得到LrCMO基因全长cDNA,并对其进行了基因表达分析。结果表明:LrCMO基因全长1 472 bp,其中开放阅读框(ORF)为1 281 bp,编码427个氨基酸残基,预测编码蛋白质的分子量为47.92 kD,理论等电点为5.72;LrCMO是一个稳定的疏水蛋白,不具有跨膜结构,含有叶绿体导肽;LrCMO基因编码的氨基酸与植物其他CMO蛋白具有较高的一致性,且与海枣PdCMO、香蕉MaCMO及油棕EgCMO亲缘关系最高,聚为一类。实时荧光定量PCR分析表明,LrCMO在根、鳞茎和叶片中有表达,且在鳞茎中的表达量最高;LrCMO受聚乙二醇(PEG)处理的诱导表达,其基因相对表达量在处理后12 h达到最高。随着处理时间的延长,LrCMO基因相对表达量逐渐下调至对照水平。
[Abstract]:The full-length cDNA, of LrCMO gene was cloned from Lycoris (Lycoris radiata) by homologous cloning and RACE assay and its gene expression was analyzed. The results showed that the total length of LrCMO gene was 1 472 bp, and the open reading frame (ORF) encoded 427 amino acid residues. The predicted molecular weight of the encoded protein was 47.92 kD, and the theoretical isoelectric point was 5.72 kD, which was a stable hydrophobic protein with no transmembrane structure. The amino acids encoded by LrCMO gene were consistent with other plant CMO proteins and had the highest relationship with PdCMO, banana MaCMO and oil palm EgCMO. Real-time fluorescence quantitative PCR analysis showed that LrCMO was expressed in roots, bulbs and leaves, and the highest expression level was found in bulbs, and the relative expression of LrCMO was the highest in 12 h after treatment with polyethylene glycol (PEG). With the extension of treatment time, the relative expression of LrCMO gene decreased gradually to the control level.
【作者单位】： 江苏省中国科学院植物研究所;
【基金】：国家自然科学基金资助项目(31301798) 江苏省第四期“333”工程培养资金资助项目(BRA2015432) 江苏省省属公益院所科研条件与能力建设项目(BM2015019)共同资助
【分类号】：Q943.2;S682.29
本文编号：2267993
[Abstract]:The full-length cDNA, of LrCMO gene was cloned from Lycoris (Lycoris radiata) by homologous cloning and RACE assay and its gene expression was analyzed. The results showed that the total length of LrCMO gene was 1 472 bp, and the open reading frame (ORF) encoded 427 amino acid residues. The predicted molecular weight of the encoded protein was 47.92 kD, and the theoretical isoelectric point was 5.72 kD, which was a stable hydrophobic protein with no transmembrane structure. The amino acids encoded by LrCMO gene were consistent with other plant CMO proteins and had the highest relationship with PdCMO, banana MaCMO and oil palm EgCMO. Real-time fluorescence quantitative PCR analysis showed that LrCMO was expressed in roots, bulbs and leaves, and the highest expression level was found in bulbs, and the relative expression of LrCMO was the highest in 12 h after treatment with polyethylene glycol (PEG). With the extension of treatment time, the relative expression of LrCMO gene decreased gradually to the control level.
【作者单位】： 江苏省中国科学院植物研究所;
【基金】：国家自然科学基金资助项目(31301798) 江苏省第四期“333”工程培养资金资助项目(BRA2015432) 江苏省省属公益院所科研条件与能力建设项目(BM2015019)共同资助
【分类号】：Q943.2;S682.29
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