黄牡丹PlWDR3和PlWDR18转录因子基因的克隆与表达

发布时间：2018-10-13 15:21

【摘要】：以云南野生黄牡丹为试验材料,根据已构建的云南野生黄牡丹花瓣转录组数据库提供的WDR40蛋白的Unigene筛选得到19个与花色素合成相关的WDR40蛋白同源性高的Unigene序列,命名为PlWDR1~19.通过氨基酸序列比较和系统进化树分析,发现PlWDR3和PlWDR18可能参与调控黄牡丹的花色素合成。PlWDR3的ORF包含1个1 032bp的开放阅读框,编码1个344个氨基酸的蛋白,与苹果MdTTG1的亲缘关系最近,相似性达83.29%;PlWDR18的ORF包含1个1 035bp的开放阅读框,编码1个345个氨基酸的蛋白,与葡萄VvWDR2相似性最高,达93.77%。相对荧光定量PCR分析表明,PlWDR3在黄牡丹和紫牡丹的不同时期的表达模式基本相似,在黄牡丹和紫牡丹圆桃期表达量最高,在初开期表达量最低;PlWDR18在黄牡丹花发育初期呈上升趋势,在透色期达到最高峰,然后下降,在初开期达到最小值;在紫牡丹花发育初期亦呈上升趋势,在圆桃期达到最大值,然后逐渐下降,在盛开期降到最小值。PlWDR18在黄牡丹和紫牡丹花的各个发育时期的表达量均高于PlWDR3的表达量。推测PlWDR3和PlWDR18参与黄牡丹花色形成的调控,为今后深入探讨黄牡丹花色形成机制奠定基础。
[Abstract]:Using Yunnan wild yellow peony as experimental material, 19 Unigene sequences with high homology of WDR40 protein related to anthocyanin synthesis were obtained according to the Unigene screening of WDR40 protein provided by the established transcriptional database of petals of Yunnan wild yellow peony, and named PlWDR1~19.. By amino acid sequence comparison and phylogenetic tree analysis, it was found that PlWDR3 and PlWDR18 may be involved in the regulation of anthocyanin synthesis in Peony. The ORF of PlWDR3 contains an open reading frame of 1 032bp and encodes a 344-amino acid protein. The ORF with the similarity of 83.29 and PlWDR18 contains an open reading frame of 1 035bp and encodes a protein of 1 345 amino acids, which is the most similar to the grape VvWDR2, reaching 93.77. Relative fluorescence quantitative PCR analysis showed that the expression patterns of PlWDR3 in different stages of yellow peony and purple peony were basically similar, the expression level of PlWDR3 was the highest in yellow peony and purple peony round peach, and the lowest in the initial opening stage, and PlWDR18 showed an upward trend in the early development of yellow peony. It reached the highest peak in the permeation period, then decreased, and reached the minimum value in the initial blooming stage, and increased in the early stage of the development of purple peony, reached the maximum value in the round peach stage, and then decreased gradually. The expression of PlWDR18 in yellow peony and purple peony was higher than that of PlWDR3. It was speculated that PlWDR3 and PlWDR18 were involved in the regulation of yellow peony color formation, which laid a foundation for further study on the mechanism of yellow peony color formation.
【作者单位】：西北农林科技大学风景园林艺术学院;中国林业科学研究院林业研究所国家林业局林木培育重点实验室;
【基金】：国家“863”计划(2011AA10020701) 西北农林科技大学2015年博士科研启动项目(Z109021611)
【分类号】：S685.11

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