玉米SNAC基因的遗传变异及耐旱性调控

发布时间：2018-10-14 16:33

【摘要】：以我国玉米育种中常用的16份自交系为材料,通过对SNAC(Stress-responsive NAM,ATAF1/2,CUC2)基因编码区及上游启动子区800 bp核苷酸序列进行测序,检测SNAC基因在不同杂种优势类群材料中的遗传变异。在12个SNAC基因中,其中有4个基因在上游800 bp区检测到遗传变异,有4个基因变异位点超过30个,多态性较高。虽然大多数SNAC基因变异以SNP(Single nucleotide polymorphism)为主,但在ZmNAC031467基因中检测到较多的插入缺失变异(In Del),达到基因总遗传变异的63.3%。通过PLACE软件对上游启动子有变异的4个基因进行3种耐逆结合元件的预测,结果显示4个基因均含有3种耐逆结合元件,但是基因突变对启动子结合元件的影响较小。再对检测到的遗传变异进行核苷酸多态性分析和中性检验,有7个SNAC基因核苷酸多态性较高,其中Zm NAC080308基因的多态性达到0.00962,推测这些基因在遗传漂移过程中受自然选择影响较大。利用t检验初步发现Zm NAC070395和Zm NAC080398基因的2个变异位点与耐旱相关性状关联,为进一步分析SNAC基因核苷酸变异与耐旱性状的关系提供一定的借鉴。
[Abstract]:Sixteen inbred lines commonly used in maize breeding in China were used to detect the genetic variation of SNAC gene in different heterosis groups by sequencing the 800 bp nucleotide sequences in the coding region and upstream promoter region of SNAC (Stress-responsive NAM,ATAF1/2,CUC2) gene. Of the 12 SNAC genes, 4 were found to have genetic variations in the upstream 800 bp region, and 4 had more than 30 mutation sites, with high polymorphism. Although the majority of SNAC gene mutations were SNP (Single nucleotide polymorphism), more insertion deletion mutations (In Del),) were detected in the ZmNAC031467 gene than in the total genetic variation (63.3%). PLACE software was used to predict three kinds of stress-tolerant binding elements for four genes with mutation in upstream promoter. The results showed that all four genes contained three kinds of stress-resistant binding elements, but the effect of gene mutation on promoter binding elements was small. Nucleotide polymorphism analysis and neutral test showed that 7 SNAC genes had higher nucleotide polymorphisms. The polymorphism of Zm NAC080308 gene reached 0.00962, which suggested that these genes were greatly affected by natural selection during genetic drift. T test was used to find the correlation between the two mutation sites of Zm NAC070395 and Zm NAC080398 genes and drought tolerance traits, which provided some reference for further analysis of the relationship between nucleotide variation and drought resistance of SNAC gene.
【作者单位】：山西农业大学农学院;中国农业科学院作物科学研究所;吉林省通化市农业科学研究院;
【基金】：国家自然科学基金重大国际合作项目(31661143010)和面上项目(31271735)资助~~
【分类号】：S513

【参考文献】