油菜素内酯合成和信号转导基因在马铃薯块茎贮藏期间的表达变化及对萌芽的影响

发布时间：2018-10-18 13:30

【摘要】：为探明油菜素内酯BR在块茎萌芽中的作用,建立更有效的种薯催芽调控体系,选择了休眠期不同的3个品种,利用q RT-PCR分析与BR合成、信号转导、调控有关的9个基因在贮藏期间及抑芽处理下的表达模式;同时检测BR类似物24-表油菜素内酯(24-e BL)及其与赤霉素GA3对块茎萌芽的影响。结果表明,涉及BR合成的4个基因表达量均随贮藏时间延长升高,短休眠品种升高的时间点早于中、长休眠品种;信号转导及调控基因中BRI1和CYCD3的变化与合成基因相似,BSK和TCH4的表达量则在中、长休眠期品种中保持恒定。抑芽处理在贮藏前期能刺激这些基因的表达升高,但之后都迅速下降并保持低水平。转录因子BZR1在各品种中以及抑芽处理下均没有明显变化。24-e BL利于块茎解除休眠,但不促进芽的伸长生长,与GA3互配效果更佳,单株块茎增重37.92%~98.41%。结论表明,BR合成和信号转导是块茎从休眠向萌芽转变的必经生理过程,它与GA3互配用于催芽更利于种薯萌芽的整齐、健壮并促进块茎形成。
[Abstract]:In order to investigate the role of Brassinolide (BR) in tuber germination, a more effective regulation system of seed tuber germination was established. Three varieties with different dormancy stages were selected. Q RT-PCR analysis, BR synthesis and signal transduction were used. The expression patterns of 9 genes were regulated during storage and bud inhibition, and the effects of 24-e BL (24-e BL), a BR analogue, and gibberellin (GA3) on tuber germination were detected. The results showed that the expression of four genes involved in BR synthesis increased with the prolongation of storage time, and the increase time of short dormancy varieties was earlier than that of medium and long dormancy varieties. The changes of BRI1 and CYCD3 in signal transduction and regulation genes were similar to those in synthetic genes, while the expression of BSK and TCH4 in medium and dormant varieties remained constant. Bud inhibition treatment stimulated the expression of these genes in the early stage of storage, but then decreased rapidly and kept low level. The transcription factor BZR1 did not change significantly in all varieties and under bud inhibition treatment. 24-e BL was beneficial to the release of dormancy from tuber, but did not promote the elongation and growth of buds, and had better effect with GA3. The weight of tuber per plant increased 37.92% and 98.41%. The results showed that BR synthesis and signal transduction were the necessary physiological process of tuber transformation from dormancy to germination, and the combination of GA3 and GA3 was beneficial to the neatness of seed tubers and promote tuber formation.
【作者单位】：绵阳市农业科学研究院;四川农业大学农学院;
【基金】：现代农业产业技术体系四川薯类创新团队项目(川农业函[2014]91号) 四川省科技厅公益性育种攻关项目(2016NYZ0032) 绵阳市农业科学研究院创新基金项目(cxjj462016-2019)资助~~
【分类号】：S532

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