入侵种西花蓟马与本地近缘种花蓟马的双基因鉴定

发布时间：2018-10-19 17:35

【摘要】：快速准确的害虫种类识别是有效筛选天敌昆虫开展靶向性生物防控的关键。入侵种西花蓟马Frankliniella occidentalis与本地种花蓟马F.intonsa常同域同期同种寄主上发生,因体型小、形态相似,难以快速准确鉴定。本研究以mt DNA COI与r DNA ITS2基因为标记对我国10个采样点的西花蓟马和花蓟马开展分子鉴定研究。结果显示,当以COI或ITS2基因为靶标时,西花蓟马与花蓟马间的平均遗传距离分别为0.221和0.113,是种内遗传距离的22.1和18.8倍,且种内、种间遗传距离间无重叠,并在系统发育树上聚为独立的分支,表明2种基因均可准确区分西花蓟马和花蓟马。此外,基于COI基因构建的系统发育树,西花蓟马的各单倍型聚为了2个亚分支,并分别对应温室品系和羽扇豆品系,表明COI基因还可用于西花蓟马品系的识别鉴定。研究结果对近缘种花蓟马的快速鉴定、专食性天敌昆虫的筛选,及其生防效果评价具有重要意义。
[Abstract]:Rapid and accurate identification of insect species is the key to screening natural enemy insects to carry out targeted biological control. The invasion species Frankliniella occidentalis and the native thrips F.intonsa occur in the same homologous region at the same time. Because of their small size and similar morphology, it is difficult to identify them quickly and accurately. In this study, the molecular identification of western flower thrips and flower thrips from 10 sampling sites in China was carried out using mt DNA COI and r DNA ITS2 as markers. The results showed that the average genetic distances between thrips and thrips were 0.221 and 0.113 respectively, which were 22.1 times and 18.8 times as much as those within species when COI or ITS2 was used as the target, and there was no overlap between the genetic distances within species and between species. The phylogenetic tree is an independent branch, indicating that the two genes can accurately distinguish thrips from thrips. In addition, the phylogenetic tree constructed based on COI gene showed that the haplotypes of thrips were divided into two subbranches, corresponding to greenhouse strain and Lupan strain, respectively, which indicated that COI gene could also be used for identification and identification of thrips spp. The results of this study are of great significance to the rapid identification of related thrips, the screening of specific natural enemies, and the evaluation of their biological control effects.
【作者单位】：中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室;北京市植物保护站;农业部外来入侵生物预防与控制研究中心;
【基金】：国家重点研发计划(2017YFC1201200,2016YFC1201200) 国家自然科学基金(31572067) 农业部“948”计划(2016-X48) 中国农业科学院科技创新工程项目
【分类号】：S433.89

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