层粘连蛋白基因家族在猪多能干细胞中的表达谱分析

发布时间：2018-10-22 09:57

【摘要】：层粘连蛋白(Laminin)是细胞外基质的重要成分,对细胞生长、分化、运动、组织修复和再生等发挥重要调节作用。Laminin包括有A1-5、B1-4和C1-3共12个编码基因,以不同的表达模式发挥功能。其中Laminin A5作为可以支持多能细胞生长的重要基因,得到广泛研究。但是,在所有猪相关数据库中,均未能查到Laminin A5的信息。文中通过生物信息学分析,首次确认了猪Laminin A5的存在,并进行了克隆和测序验证。为揭示Laminin基因家族在猪诱导多能干细胞(i PSCs)中的表达特性,检测了Laminin在猪各组织、体细胞和i PS细胞中的不同表达模式。发现Laminin B1基因在猪多能干细胞中存在特异性可变剪接体(LAMB1-a),且该可变剪切体的表达量与猪多能干细胞的重编程程度呈正相关。为进一步揭示和利用Laminin作为细胞外基质,用于猪多能干细胞的获取和培养优化奠定了基础。
[Abstract]:Laminin (Laminin), an important component of extracellular matrix, plays an important role in regulating cell growth, differentiation, movement, tissue repair and regeneration. Laminin includes 12 coding genes, A1-5, B1-4 and C1-3, which function in different expression patterns. Laminin A 5, as an important gene supporting pluripotent cell growth, has been widely studied. However, no Laminin A 5 information was found in all pig databases. By bioinformatics analysis, the existence of pig Laminin A 5 was confirmed for the first time, and cloned and sequenced. In order to reveal the expression characteristics of Laminin gene family in porcine induced pluripotent stem cells (i PSCs), different expression patterns of Laminin were detected in porcine tissues, somatic cells and I PS cells. It was found that Laminin B1 gene had a specific variable splicing body (LAMB1-a) in porcine pluripotent stem cells, and the expression of this variable splice body was positively correlated with the degree of reprogramming of porcine pluripotent stem cells. The results laid a foundation for further revealing and using Laminin as extracellular matrix for the acquisition and culture optimization of porcine pluripotent stem cells.
【作者单位】：西北农林科技大学动物医学院动物生物技术系陕西省干细胞工程技术研究中心;
【基金】：国家自然科学基金(Nos.31571521,31371505)资助~~
【分类号】：Q23

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