干旱胁迫下‘宁玉’草莓生长发育及相关基因表达特点

发布时间：2018-10-24 16:46

【摘要】：为分析基因诊断草莓在干旱逆境条件下生长状态的可行性,以‘宁玉’草莓为试材,调查了不同干旱胁迫条件下的生长特点以及与生长素合成、脱落酸合成、花果发育、色素合成相关的10个基因的表达情况。研究结果表明:在不同干旱条件下草莓均可完成从营养生长到生殖生长的生命周期,所选基因也都行使并完成了其调控功能。与对照植株相比,在轻度和中度干旱胁迫(50%~65%土壤持水量)条件下,草莓植株的物候期较对照(80%土壤持水量)提早,而在严重干旱胁迫(35%土壤含水量时)条件下延缓,随着干旱胁迫条件的加重,草莓植株、叶片、果实明显变小,果实着色加深。每个基因在不同干旱条件下表达水平的变化趋势基本一致,且都与相应的物候期以及生长发育时间长短的变化情况相一致,但所选基因在几种干旱条件下其表达开始的早晚、表达水平的高低以及表达相应程度持续时间的长短存在不同。在轻度和中度干旱条件下,这些基因的表达时间提早,但持续时间较短,表达水平高于对照;而在重度干旱条件下,这些基因的表达开始的时间较对照延缓,表达水平较低但持续时间拉长。上述研究结果表明,利用基因的表达信息可以提前推断草莓植株在不同干旱条件下的生长发育状态,了解干旱胁迫对草莓生长发育的影响以及为是否需要提早采取管理措施等提供重要依据。
[Abstract]:In order to analyze the feasibility of genetic diagnosis of strawberry growth under drought stress, the growth characteristics of 'Ningyu' strawberry under different drought stress conditions were investigated, and the synthesis of auxin, the synthesis of abscisic acid and the development of flower and fruit were investigated. Expression of 10 genes related to pigment synthesis. The results showed that strawberry could complete the life cycle from vegetative growth to reproductive growth under different drought conditions, and all the selected genes performed their regulatory functions. The phenological period of strawberry plants was earlier than that of control plants under mild and moderate drought stress (50% and 65% soil water holding capacity), but delayed under severe drought stress (35% soil water content). With the increase of drought stress, strawberry plants, leaves and fruits became smaller and the fruit coloration deepened. The trend of expression level of each gene was basically the same under different drought conditions, and was consistent with the change of phenological period and growth and development time, but the expression of selected gene began in the morning and evening under several drought conditions. The level of expression and the duration of the corresponding degree of expression are different. In mild and moderate drought conditions, the expression of these genes was earlier, but the duration was shorter, and the expression level was higher than that of the control, while in severe drought, the time of expression of these genes was slower than that of the control. The expression level is low but the duration is long. The results showed that the growth and development of strawberry plants under different drought conditions could be predicted in advance by using the gene expression information. To understand the effects of drought stress on strawberry growth and development and to provide important basis for early adoption of management measures.
【作者单位】：南京农业大学园艺学院;
【基金】：国家自然科学基金项目(31401847) 江苏省自然科学基金项目(BK20140707) 国家博士后基金项目(2014M561663) 中央高校基本科研业务费专项资金项目(KJQN201541)
【分类号】：S668.4

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