微胶囊饲料对马氏珠母贝育珠性能、生长基因和矿化基因表达的影响

发布时间：2018-10-26 11:17

【摘要】：本研究分析了自主研发的微胶囊饲料替代微藻对马氏珠母贝(Pinctada fucata martensii)育珠性能、生长相关基因和矿化相关基因表达的影响。共设置了3个实验组,其中,EG1组投喂亚心形扁藻(Platymonas subcordiformis),EG2组投喂微胶囊饲料+亚心形扁藻,EG3组投喂微胶囊饲料。养殖170 d后,比较马氏珠母贝的育珠性能、闭壳肌生长相关基因EGFR、FGF18、GHITM和TβRI以及外套膜的中央膜和边缘膜矿化相关基因pearlin、DPT、pif177和N19的表达。结果表明:(1)各组间马氏珠母贝育珠贝的存活率、留核率不存在显著性差异(P0.05),所采收珍珠的珍珠层厚度及平均质量也不存在显著性差异(P0.05);(2)各实验组间马氏珠母贝闭壳肌中EGFR、FGF18、GHITM和TβR I的相对表达量不存在显著性差异(P0.05);(3)各实验组间马氏珠母贝中央膜中DPT和N19的相对表达量差异不显著(P0.05),EG2和EG3组pearlin的表达量显著性高于EG1组(P0.05),pif177在EG3组中的表达量显著性高于EG1和EG2组(P0.05);(4)各实验组间马氏珠母贝边缘膜中pearlin、DPT和N19的相对表达量差异不显著(P0.05),EG2和EG3组pif177的表达量显著高于EG1组(P0.05)。研究结果说明,该微胶囊饲料可以替代部分微藻进行育珠生产,为进一步研发马氏珠母贝的人工配合饲料及开展工厂化育珠积累了参考资料。
[Abstract]:In this study, the effects of microencapsulated feed substitute microalgae on the performance of (Pinctada fucata martensii) beads, the expression of growth-related genes and mineralization-related genes were studied. Three experimental groups were established, among which the EG1 group was fed with microencapsulated feed of (Platymonas subcordiformis), EG2 and the EG3 group was fed with microencapsulated feed. After 170 days of culture, the bead fertility, the expression of growth related genes EGFR,FGF18,GHITM and T 尾 RI of the obturator muscle, and the expression of the mineralization-related genes pearlin,DPT,pif177 and N19 in the central membrane and marginal membrane of the outer membrane were compared. The results showed that: (1) there was no significant difference in the survival rate and the rate of nuclear retention among groups (P0.05), and there was no significant difference in the thickness and average mass of pearl (P0.05). (2) there was no significant difference in the relative expression of EGFR,FGF18,GHITM and T 尾 RI between the experimental groups (P0.05); (3) there was no significant difference in the relative expression of DPT and N19 in the central membrane of Pinctada martensii among the experimental groups (P0.05). The expression of pearlin in the EG2 and EG3 groups was significantly higher than that in the EG1 group (P0.05). The expression of pif177 in EG3 group was significantly higher than that in EG1 and EG2 group (P0.05). (4) there was no significant difference in the relative expression of pearlin,DPT and N19 between each experimental group (P0.05), but the expression of pif177 in EG2 and EG3 group was significantly higher than that in EG1 group (P0.05). The results showed that the microencapsulated feed could replace some microalgae for bead production, which provided a reference for the further research and development of artificial feed and industrial production of the pellet of Pinctada martensii.
【作者单位】：广东海洋大学水产学院;广东省珍珠养殖与加工工程技术研究中心;
【基金】：广东省科技计划项目(2014A020208122) 国家贝类产业体系专项(CRAS-048) 广东省海洋渔业科技与产业发展专项(Z2014009;A201308A06)
【分类号】：S968.31

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