2型糖尿病合并非酒精性脂肪肝中医体质分布规律与基因多态性关系研究

发布时间：2018-10-29 15:37

【摘要】：目的:探讨2型糖尿病(T2DM)合并非酒精性脂肪肝(NAFLD)患者中医体质分布规律及其与脂联素基因(ap M1)多态性的关系。方法:在184例宁波地区汉族人群(T2DM合并NAFLD95例,健康人群89名)中,采用PCR技术研究脂联素45位点的单核苷酸多态性(SNP),并运用体质九分法进行中医体质分型,描述其分布频数比,并用逻辑回归法分析中医体质与基因多态性的关系。结果:宁波地区汉族人群T2DM合并NAFLD患者中,ap M1+45有T/G的SNP存在,其等位基因分布与健康人群有显著差异(P0.05),突变型(T/G、G/G)人群该病易感性增加(OR=1.947,95%CI[1.082,3.503])。NAFLD组人群中气虚质(46.3%)、痰湿质(32.6%)分布增多,与健康人群比较有显著差异(P0.05),且气虚、痰湿质分布与突变型(T/G、G/G)的出现呈正相关。结论:脂联素基因+45位点T/G、G/G基因突变型人群T2DM合并NAFLD的易感性增加,随着突变型(T/G、G/G)分布增多,气虚、痰湿体质出现亦增多。
[Abstract]:Objective: to investigate the distribution of TCM constitution in type 2 diabetes mellitus (T2DM) with non-alcoholic fatty liver (NAFLD) and its association with adiponectin gene (ap M1) polymorphism. Methods: the single nucleotide polymorphism (SNP),) of adiponectin 45 locus was studied by PCR technique in 184 Han population (T2DM combined with NAFLD95, 89 healthy people) in Ningbo area. The distribution frequency ratio was described and the relationship between TCM physique and gene polymorphism was analyzed by logical regression method. Results: in Ningbo Han population with T2DM and NAFLD, ap M1 45 had T / G SNP, and its allele distribution was significantly different from that of healthy population (P0.05). G / G) increased susceptibility to the disease (OR=1.947,95%CI [1.082 卤3.503]) in the). NAFLD group (46.3%), phlegm dampness (32.6%) increased, there was a significant difference compared with the healthy population (P0.05). The distribution of qi deficiency and phlegm dampness was positively correlated with the occurrence of the mutation type (T / G / G). Conclusion: the susceptibility of T2DM combined with NAFLD increased in the 45 locus of adiponectin gene. With the increase of the distribution of T / G G / G, deficiency of qi and phlegm dampness constitution were also increased.
【作者单位】：浙江中医药大学附属宁波中医院;浙江中医药大学;
【基金】：浙江省科技计划项目(No.2015C33169) 浙江省中医药科技计划项目(No.2013ZB111) 宁波市中医重点学科中西医结合内分泌科(No.甬卫发[2016]55号)~~
【分类号】：R259

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