绵羊LDHβ基因的生物信息学分析及其在不同剩余采食量绵羊肝脏和肌肉组织中的表达

发布时间：2018-10-30 07:37

【摘要】：【目的】为鉴定绵羊乳酸脱氢酶β(lactate dehydrogenase B,LDHβ)基因的分子特征,研究其在不同剩余采食量绵羊肝脏和肌肉组织中的表达差异.【方法】测定了137只‘湖羊’公羔的剩余采食量(residual feed intake,RFI),按RFI进行排序,分别筛选出RFI最高(high-residual feed intake,H-RFI)和RFI最低(low-residual feed intake,L-RFI)的羊各15只,屠宰后,采集肝脏和肌肉组织,利用Q-PCR技术检测绵羊LDHβ基因分别在H-RFI和L-RFI羊肝脏和肌肉中表达量,并利用生物信息学软件构建了该基因的系统进化树和预测其结构与功能.【结果】绵羊LDHβ基因开放阅读框(open reading frame,ORF)为1 005bp,编码334个氨基酸,其蛋白质分子质量为36 479.43U,理论等电点为6.40.绵羊LDHβ基因与山羊的亲缘关系较近,其次为牛,序列同源性高.功能结构域预测结果显示,该基因编码产物在内质网中参与辅酶因子生物合成的可能性最高.Q-PCR结果显示,绵羊LDHβ-mRNA基因在L-RFI羊肝脏和肌肉中表达量均显著低于H-RFI羊(P0.01).【结论】绵羊LDHβ基因作为能量代谢的关键酶参与绵羊饲料效率的调控.
[Abstract]:[objective] to identify the molecular characteristics of lactate dehydrogenase (lactate dehydrogenase) 尾 (lactate dehydrogenase 尾 gene in sheep. To study the difference of its expression in liver and muscle tissues of sheep with different residual feed intake. [methods] the residual feed intake (residual feed intake,RFI) of 137 male lambs of Hu sheep was determined and sorted by RFI. Fifteen sheep with the highest RFI (high-residual feed intake,H-RFI) and the lowest RFI (low-residual feed intake,L-RFI) were selected respectively. After slaughtering, liver and muscle tissues were collected. Q-PCR technique was used to detect the expression of LDH 尾 gene in liver and muscle of H-RFI and L-RFI sheep, respectively. The phylogenetic tree of the gene was constructed by bioinformatics software, and its structure and function were predicted. [results] the open reading frame of sheep LDH 尾 gene (open reading frame,ORF) was 1 005 BP, encoding 334 amino acids. The molecular weight of the protein is 36,479.43 U and the theoretical isoelectric point is 6.40. Sheep LDH 尾 gene was closely related to goats, followed by cattle, with high sequence homology. The predicted results of functional domain showed that the gene encoding product was most likely to participate in coenzyme factor biosynthesis in the endoplasmic reticulum. Q-PCR results showed that the gene encoding product was more likely to participate in coenzyme factor biosynthesis. The expression of LDH 尾-mRNA gene in liver and muscle of L-RFI sheep was significantly lower than that in H-RFI sheep (P0.01). [conclusion] Sheep LDH 尾 gene is involved in the regulation of feed efficiency as a key enzyme of energy metabolism.
【作者单位】：甘肃农业大学动物科学技术学院;甘肃省肉羊繁育生物技术工程实验室;
【基金】：国家自然科学基金项目(31560625) 现代农业产业技术体系建设专项(CARS-39)
【分类号】：S826

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