枇杷脱水素基因EjDHN5抗低温胁迫功能研究
发布时间：2018-11-02 19:30
【摘要】：为研究枇杷(Eriobotrya japonica Lindl.)脱水素基因(Ej DHN5)在枇杷抗低温胁迫中的功能,将其在烟草中进行过量表达,其中表达水平比较高的两个株系L24和L26被用来进行功能研究。野生型和转基因烟草苗分别用0、2、4和8μmol·L~(-1)的甲基紫精(Methyl viologen,自由基发生剂)处理并进行光照培养,结果显示,4和8μmol·L~(-1)甲基紫精处理使野生型烟草苗存活率分别下降到73.6%和56.9%,光系统Ⅱ(PSⅡ)活性显著降低,而转基因苗的存活率仍维持在100%,PSⅡ活性明显高于野生型。对六叶期烟草的叶盘进行甲基紫精处理,野生型烟草叶盘的叶绿素含量显著低于转基因株系,而活性氧含量和MDA含量明显高于转基因株系。对野生型和转基因烟草苗进行低温处理,转基因株系生长状况明显优于野生型,活性氧含量低于野生型,膜质过氧化程度轻于野生型。这些结果表明,过量表达EjDHN5能提高烟草的抗氧化胁迫和抗低温胁迫能力,推测EjDHN5在提高枇杷抗低温能力方面起着重要作用可能与其能提高抗氧化胁迫能力有关。
[Abstract]:Study on loquat (Eriobotrya japonica Lindl.) The function of dehydrin gene (Ej DHN5) in loquat resistance to low temperature stress was overexpressed in tobacco. Two lines L24 and L26 with higher expression level were used to study their function. Wild type and transgenic tobacco seedlings were treated with (Methyl viologen, free radical generator of 0 and 8 渭 mol L ~ (-1), respectively, and cultured under light. The survival rate of wild type tobacco seedlings decreased to 73.6% and 56.9%, respectively, and the activity of PS 鈪，
本文编号：2306749
[Abstract]:Study on loquat (Eriobotrya japonica Lindl.) The function of dehydrin gene (Ej DHN5) in loquat resistance to low temperature stress was overexpressed in tobacco. Two lines L24 and L26 with higher expression level were used to study their function. Wild type and transgenic tobacco seedlings were treated with (Methyl viologen, free radical generator of 0 and 8 渭 mol L ~ (-1), respectively, and cultured under light. The survival rate of wild type tobacco seedlings decreased to 73.6% and 56.9%, respectively, and the activity of PS 鈪，
本文编号：2306749
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