安徽农村地区HIV感染者和幼儿园儿童蓝氏贾第鞭毛虫感染情况及其基因型

发布时间：2018-11-06 07:40

【摘要】：目的了解和比较安徽农村地区人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者和幼儿园儿童中蓝氏贾第鞭毛虫(Giardia lamblia)感染情况及其基因型。方法2015年4月24日至5月9日收集安徽农村地区某艾滋病治疗点登记在册的HIV感染者及当地一家幼儿园所有儿童的相关信息,并采集粪样进行碘液染色及镜检。提取镜检阳性者的粪样DNA,采用巢式PCR扩增蓝氏贾第鞭毛虫磷酸丙糖异构酶(triosephosphate isomerase,tpi)基因并测序,通过BLAST、Clustal X 1.83和MEGA 6.0软件对基因序列进行同源性比较和系统发育分析。结果共调查HIV感染者127人和儿童125人,发现贾第鞭毛虫感染者4例,其中儿童3例,HIV感染者1例,感染率分别为2.40%(3/125)和0.79%(1/127),两者差异无统计学意义(P0.05)。3例儿童感染者粪便为成形软便,无腹泻、腹痛等临床症状;1例HIV感染者粪便为稀便,且有明显腹痛、腹泻、乏力症状,体重下降明显。PCR检测结果显示,4例感染者粪样DNA扩增产物均在约500 bp处出现特异条带。测序结果显示,4例均感染蓝氏贾第鞭毛虫,其中HIV感染者2个重复样品的序列相似性为79%,与已报道的上海人源贾第鞭毛虫分离株(Gen Bank登录号:KF271445)相似性分别为79%、98%;3名儿童感染者的样本序列相似性达99%,与上海人源贾第鞭毛虫分离株相似性均为79%。以tpi基因构建的系统进化树结果显示,HIV感染者的蓝氏贾第鞭毛虫分离株为复合型集聚体A+B,3名儿童感染者分离株均为集聚体A;HIV感染者的集聚体A与3名儿童感染者分离株的同源性较高。结论安徽农村地区HIV感染者和儿童人群中存在蓝氏贾第鞭毛虫感染,HIV感染者的虫株基因型为复合型集聚体A+B,儿童的虫株基因型皆为集聚体A。
[Abstract]:Objective to investigate and compare the (Giardia lamblia) infection and genotype of Giardia lamblia in rural areas of Anhui province. Methods from April 24 to May 9, 2015, we collected the relevant information of HIV infected persons registered at an AIDS treatment site in rural Anhui and all children in a local kindergarten, and collected feces for iodine staining and microscopic examination. Fecal DNA, was extracted from positive individuals. Nested PCR was used to amplify and sequence the triosephosphate isomerase,tpi gene of Giardia lamblia. Clustal X 1.83 and MEGA 6.0 were used to compare the homology and phylogenetic analysis. Results A total of 127 cases of HIV infection and 125 cases of children were investigated. Four cases of Giardia lamblia infection were found, including 3 cases of children and 1 case of HIV infection. The infection rate was 2.40% (3 / 125) and 0.79% (1 / 127), respectively. There was no significant difference between the two groups (P0.05). The feces of 3 children infected with infection were soft stool, no diarrhea, abdominal pain and other clinical symptoms. The feces of one case of HIV infection were dilute stool with obvious abdominal pain, diarrhea, fatigue and weight loss. The results of PCR detection showed that the fecal DNA amplification products of 4 cases of HIV infected patients had specific bands at about 500 bp. The sequencing results showed that all the 4 cases were infected with Giardia lamblia. The sequence similarity of two repeat samples of HIV infection was 79, and the similarity was 79 with the reported (Gen Bank accession number: KF271445 of Shanghai Giardia lamblia isolates. 98. The similarity of the three infected children was 99g, and the similarity with Shanghai Giardia flagellate isolate was 79g. The results of phylogenetic tree constructed by tpi gene showed that the isolates of Giardia lambtii infected with HIV were compound agglomeration A BU. All the isolates of 3 children infected with HIV were agglomerates. There was a high homology between the agglomeration A of HIV infection and the isolated strains of 3 children infected with HIV. Conclusion there is Giardia lamblia infection in HIV infected persons and children in rural areas of Anhui province. The genotype of HIV infected individuals is compound agglomeration A B, and that of children is agglomeration A.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所世界卫生组织热带病合作中心科技部国家级热带病国际联合中心卫生部寄生虫病原与媒介生物学重点实验室;
【基金】：国家自然科学基金(No.81473022) 上海市卫计委科研课题(No.140826120622123)~~
【分类号】：R725

【相似文献】