蜕皮激素受体和超气门蛋白基因在柞蚕发育过程及激素诱导后的表达模式

发布时间：2018-11-06 08:23

【摘要】：昆虫蜕皮激素受体和超气门蛋白形成的异源二聚体介导蜕皮激素信号,起始包括转录因子表达在内的蜕皮级联反应,从而使昆虫进入蜕皮和变态等生命过程。克隆了柞蚕的2个蜕皮激素受体相关基因,命名为Ap EcRB1(Gen Bank登录号:KY411159)和Ap USP1(Gen Bank登录号:KY411160)。2个基因的ORF序列全长分别为1 758 bp、1 401 bp,编码585个、466个氨基酸。半定量RT-PCR检测这2个基因除在柞蚕幼虫血淋巴中不表达之外,在其他组织中均有表达,在丝腺和脂肪体中的表达量较高。实时荧光定量PCR(qRT-PCR)分析2个基因在幼虫蜕皮前及化蛹前后呈现大幅度上调表达的规律。注射20-羟基蜕皮甾酮(20E)诱导启动滞育蛹的发育,结果诱导组的滞育蛹经过17 d羽化,比对照组滞育蛹提早羽化。滞育蛹中2个基因的表达量在注射20E后均明显下降,其中Ap EcRB1在蛹发育后期的12 d内一直处于较低水平,但羽化前期其表达量急剧上升,蛹发育16 d(羽化前1 d)达到最高;Ap USP1基因的表达量从注射后8 d开始升高,至16 d时达到最高。以上研究结果丰富了柞蚕变态发育过程中蜕皮激素调控作用的分子信息。
[Abstract]:Ecdysone receptors and metameric proteins mediate ecdysone signaling, which initiate ecdylastic cascade reactions, including transcription factor expression, leading to ecdymids and metamorphosis. Two ecdysone receptor related genes of Antheraea pernyi were cloned, named Ap EcRB1 (Gen Bank accession number: KY411159) and Ap USP1 (Gen Bank accession number: KY411160). The ORF sequences of the two genes were 1 758 bp,1 401 bp, encoding 585, respectively. 466 amino acids. The two genes were detected by semi-quantitative RT-PCR, except in the hemolymph of Antheraea pernyi larvae, they were expressed in other tissues, and were higher in the silk gland and fat body. Real-time fluorescence quantitative PCR (qRT-PCR) was used to analyze the expression of two genes in larva before molting and before and after pupae formation. The development of diapause pupae was induced by injection of 20-hydroxyl ecdysterone (20E). Results the diapause pupae in the induced group was ejaculated after 17 days, which was earlier than that in the control group. The expression of two genes in diapause pupae decreased significantly after injection of 20e. The expression of Ap EcRB1 was at a low level during the 12 days of pupae development, but the expression level increased sharply in the early stage of emergence, and reached the highest level in the 16th day (1 day before emergence) of pupae development. The expression of Ap USP1 gene increased from 8 days after injection to the highest level at 16 days. These results enrich the molecular information of ecdysone regulation during antheraea pernyi metamorphosis.
【作者单位】：沈阳农业大学生物科学技术学院辽宁省昆虫资源工程技术研究中心;黑龙江蚕蜂技术指导总站;
【基金】：现代农业产业技术体系建设专项(No.CARS-22) 辽宁省农业领域青年科技创新人才培养计划项目(No.201401-0) 辽宁省教育厅一般项目(No.L2015488)
【分类号】：S885.1

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