6-姜烯酚诱导人结直肠癌细胞凋亡及与Bax、BCL2、Caspase3和PARP1基因表达的影响

发布时间：2018-11-07 19:03

【摘要】：本文通过体外细胞实验应用6-姜烯酚对HCT116和HT29进行干预,以探讨6-姜烯酚诱导结直肠癌细胞凋亡及与相关凋亡蛋白(Bax、BCL2、Caspase3和PARP1)表达之间的关系。利用倒置荧光显微镜观察不同浓度的6-姜烯酚干预HCT116及HT29后细胞形态的变化,CCK8法测定不同分组HCT116及HT29抑制率,并绘制细胞增殖曲线,Annexin V-FITC/PI流式细胞术检测不同分组HCT116及HT29凋亡,Western-blot检测分析凋亡相关蛋白表达情况(Bax、BCL2、Caspase3和PARP1)。结果显示,与对照组相比,6-姜烯酚可不同程度地诱导HCT116及HT29凋亡,促进Bax、Caspase3和PARP1蛋白水平表达(p0.05),抑制BCL2蛋白水平表达(p0.05),并增加Bax/BCL2比值比例(p0.05)。因此,6-姜烯酚诱导HCT116、HT29发生凋亡可能是与激活Bax、Caspase3和PARP1的表达以及增加Bax/BCL2比值比例相关,这一发现可为结直肠癌的临床治疗与预防提供有效参考依据。
[Abstract]:In this study, 6-gingerol was used to interfere with HCT116 and HT29 in vitro to investigate the relationship between 6-gingingenol induced apoptosis and the expression of Bax,BCL2,Caspase3 and PARP1 in colorectal cancer cells. The morphological changes of HCT116 and HT29 cells were observed by inverted fluorescence microscope. The inhibition rates of HCT116 and HT29 in different groups were measured by CCK8 method, and the cell proliferation curves were plotted. Annexin V-FITC/PI flow cytometry was used to detect HCT116 and HT29 apoptosis in different groups, and Western-blot was used to detect the expression of apoptosis-related proteins (Bax,BCL2,Caspase3 and PARP1). The results showed that 6-gingerol could induce the apoptosis of HCT116 and HT29, promote the expression of Bax,Caspase3 and PARP1 protein (p0.05), and inhibit the expression of BCL2 protein (p0.05). The ratio of Bax/BCL2 was increased (p0. 05). Therefore, the apoptosis of HCT116,HT29 induced by 6-gingerol may be related to the activation of the expression of Bax,Caspase3 and PARP1 and the increase of the ratio of Bax/BCL2, which may provide an effective reference for the clinical treatment and prevention of colorectal cancer.
【作者单位】：宁夏医科大学临床医学院;宁夏医科大学总医院;
【基金】：宁夏自然科学基金项目(NZ16136) 宁夏“研究生教育创新计划”学位点建设项目(YXW2017014)
【分类号】：R285

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