雨生红球藻PHOT的基因克隆和生物信息学分析

发布时间：2018-11-07 19:46

【摘要】：【目的】本文旨在获得雨生红球藻中蓝光受体向光素(Phototropin,PHOT)的全长序列并进行生物信息分析。【方法】采用同源克隆和RACE结合的方法获得雨生红球藻中编码HaePHOT的c DNA序列全长,并对HaePHOT进行生物信息分析。【结果】HaePHOT的开放阅读框全长为2139 bp,编码712个氨基酸,预测的等电点8.66,理论分子量78.73 k D。经BLASTP程序分析,与拟南芥来源PHOT的相似性达到58%,与莱茵衣藻来源PHOT的相似性达到68%。通过序列比对和结构域分析,PHOT蛋白的典型结构域存在于HaePHOT中,包括发色团结合和激酶结构域。系统进化分析表明,高等植物和真核绿藻来源PHOTs来自共同祖先。【结论】首次从雨生红球藻中获得编码PHOT的基因序列,为下一步雨生红球藻中PHOT的表达和功能研究奠定基础,同时为解析蓝光调控雨生红球藻虾青素合成的分子机制提供线索。
[Abstract]:[objective] to obtain blue light receptor pheromone (Phototropin,) from Haematococcus pluvialis. [methods] the full length of c DNA encoding HaePHOT in Haematococcus pluvialis was obtained by homologous cloning and RACE binding. The total length of HaePHOT open reading frame was 2139 bp, encoding 712 amino acids, the predicted isoelectric point was 8.66, and the theoretical molecular weight was 78.73 KD. By BLASTP program analysis, the similarity between PHOT and Arabidopsis PHOT was 58 and 68 with PHOT from Chlamydomonas rhinorrhoeae. By sequence alignment and domain analysis, the typical domain of PHOT protein was found in HaePHOT, including chromophore binding and kinase domain. Phylogenetic analysis showed that PHOTs from higher plants and eukaryotes originated from common ancestors. [conclusion] the gene sequence encoding PHOT was obtained for the first time from Haematococcus pluvialis, which laid a foundation for further study on the expression and function of PHOT in Haematococcus pluvialis. At the same time, it provides clues to the molecular mechanism of the regulation of astaxanthin synthesis by blue light.
【作者单位】：大连大学生命科学与技术学院;中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室;中国科学院大学;深圳大学生命与海洋科学学院;
【基金】：国家级大学生创新创业训练计划项目(201611258038) 国家自然基金项目(41376139)
【分类号】：Q943.2

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