杜梨根系铁吸收关键基因生物信息学分析及缺铁胁迫对其表达的影响

发布时间：2018-11-07 20:01

【摘要】：为了探究缺铁胁迫对杜梨根系铁吸收关键基因表达的影响,对杜梨根系铁吸收关键基因三价铁还原酶(Pb FRO2)基因和铁转运蛋白(Pb IRT1)基因的氨基酸序列进行了多重序列比对和进化树分析;采用改良的Hoagland营养液水培杜梨的方法,研究了缺铁胁迫对杜梨根系Pb FRO2和Pb IRT1基因相对表达量以及根系铁和锌含量的影响。结果表明,Pb FRO2蛋白具有还原酶特性,Pb IRT1蛋白属于二价金属离子转运蛋白家族—ZIP家族;缺铁胁迫9 d内,杜梨根系Pb FRO2和Pb IRT1的表达量整体上呈现出先升高后降低的趋势,胁迫3 d表达量达到最高点且与对照(加FeEDTA)差异显著;缺铁胁迫30 d,杜梨根系中铁含量比对照降低77.46%,而锌含量提高1 139.40%。综上可知,缺铁胁迫可诱导杜梨根系铁吸收关键基因表达量升高,进而促进二价金属离子转运蛋白的活性提高。
[Abstract]:In order to investigate the effect of iron deficiency stress on the expression of iron absorption key genes in Pyrus pyrifolia root system, The amino acid sequences of ferric reductase (Pb FRO2) gene and iron transporter (Pb IRT1) gene were analyzed by multiple sequence alignment and phylogenetic tree analysis. The effects of iron deficiency on the relative expression of Pb FRO2 and Pb IRT1 genes and the content of iron and zinc in the roots of Pyrus pyrifolia were studied by using the improved Hoagland nutrient solution. The results showed that Pb FRO2 protein had reductase properties, and Pb IRT1 protein belonged to the divalent metal ion transporter family-ZIP family. Within 9 days of iron deficiency stress, the expression of Pb FRO2 and Pb IRT1 in the roots of Pyrus pyrifolia showed a trend of first increasing and then decreasing, and the expression reached the highest point after 3 days of stress, and the difference was significant compared with that of the control (plus FeEDTA). After 30 days of iron deficiency stress, the content of iron in roots of Pyrus chinensis was 77.46 lower than that of the control, while the content of zinc increased by 1,139.40. It can be concluded that iron deficiency stress can induce the expression of iron absorption key genes in Pyrus root and promote the activity of divalent metal ion transporter.
【作者单位】：河南省农业科学院园艺研究所;
【基金】：河南省基础与前沿技术研究计划项目(162300410147) 国家梨产业技术体系项目(CARS-29-3)
【分类号】：S661.2

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