水稻非生物胁迫响应基因OsMIP1的表达与进化分析

发布时间：2018-11-08 11:28

【摘要】：MID1编码R-R型的MYB转录因子,对不同的非生物胁迫均有响应,特别是在水稻(Oryza sativa)生殖期会受到干旱胁迫的诱导,进而在一定程度上可以保持花粉的育性并稳定水稻产量。为进一步研究水稻MID1对非生物胁迫的响应网络,利用酵母双杂交系统筛选出与其互作的蛋白因子OsMIP1,并利用双分子荧光互补系统在本氏烟草(Nicotiana benthamiana)细胞中得到验证。结果表明,OsMIP1编码1个预测含有ENTH/ANTH/VHS结构域的跨膜转运蛋白。OsMIP1在根、茎、叶、小穗和胚乳中均有表达。干旱胁迫下,OsMIP1在叶片和生殖器官中表达,特别是在减数分裂后的小花中表达显著上调。这些结果暗示,OsMIP1在花器官抵抗干旱胁迫中起一定的作用。在水稻营养生长阶段,OsMIP1表达还受到包括Na Cl和甘露醇在内的其它非生物胁迫的影响,暗示其可能在其它非生物胁迫调节中也具有一定的作用。植物中关于编码ENTH/ANTH/VHS结构域蛋白的研究很少。通过对MIP1亚家族进化关系进行分析,结果表明,在被子植物中,MIP1可分为6大类,这6大类分别来自被子植物祖先中原本就存在的6个拷贝,在被子植物的进化过程中又经历了多次基因重复和拷贝丢失等事件。MIP1家族成员广泛分布于被子植物中并可能具有抗胁迫等功能。
[Abstract]:The R-R type MYB transcription factors encoded by MID1 were responsive to different abiotic stresses, especially during the reproductive period of rice (Oryza sativa), which could be induced by drought stress to a certain extent, which could maintain pollen fertility and stabilize rice yield. In order to further study the response network of rice MID1 to abiotic stress, yeast two-hybrid system was used to screen the protein factor OsMIP1, interacting with it, and the bimolecular fluorescence complementary system was used to verify it in the tobacco (Nicotiana benthamiana) cells. The results showed that OsMIP1 encodes a transmembrane transporter containing ENTH/ANTH/VHS domain. OsMIP1 is expressed in root, stem, leaf, spikelet and endosperm. Under drought stress, OsMIP1 expression was significantly up-regulated in leaves and reproductive organs, especially in meiotic florets. These results suggest that OsMIP1 may play a role in floral organ resistance to drought stress. During the vegetative growth of rice, OsMIP1 expression was also affected by other abiotic stresses, including Na Cl and mannitol, suggesting that it might also play a role in the regulation of other abiotic stresses. Little research has been done on the encoding of ENTH/ANTH/VHS domain proteins in plants. The results showed that MIP1 in angiosperms could be divided into 6 groups, which were derived from 6 copies of angiosperm ancestors. During the evolution of angiosperms, MIP1 family members are widely distributed in angiosperms and may have the function of resistance to stress.
【作者单位】：复旦大学生命科学学院;复旦大学植物科学研究所;浙江农林大学农业与食品科学学院;
【基金】：基金项目:转基因生物新品种培育(No.2014ZX08009-014B)
【分类号】：S511;Q943.2

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