基于转录组数据的蒙古牛皮肤组织抗寒相关信号通路及候选基因的筛选

发布时间：2018-11-09 11:47

【摘要】：本研究旨在通过对冬夏两季蒙古牛皮肤组织进行转录组测序和生物信息学分析,找到与蒙古牛抗寒性状相关的信号通路及候选基因。用Ion ProtonTMSequencer分别对冬季和夏季成年蒙古牛的皮肤组织进行转录组测序,随后对转录组数据进行处理、与参考基因组比对、差异表达分析、GO和KEGG富集分析以及候选基因筛选,并用Real-time PCR方法对转录组数据进行可靠性检测。结果显示,冬季组和夏季组分别得到86 954 060和69 837 009条reads;冬季组与参考基因组的唯一比对率为73.21%,夏季组为75.55%;冬夏两组共有182个差异表达基因(DEGs),与夏季组相比,其中117个在冬季组中上调,65个在冬季组中下调;Real-time PCR分析显示,转录组测序结果可靠;GO分析结果显示,细胞组分、分子功能、生物学过程分别富集到21、39和224个term;KEGG分析结果显示,差异表达基因(DEGs)显著地富集在脂类代谢、凝血、酪氨酸代谢、类固醇合成和视黄醇代谢相关通路上;共筛选出8个候选基因。综上表明,脂类代谢、凝血、黑色素合成相关通路和基因,以及与毛发发生相关的基因可能在蒙古牛抗寒过程中发挥重要作用。
[Abstract]:In this study, transcriptome sequencing and bioinformatics analysis of Mongolian cattle skin tissues in winter and summer were carried out to find the signal pathway and candidate genes related to cold resistance traits of Mongolian cattle. The skin tissues of adult Mongolian cattle in winter and summer were sequenced by Ion ProtonTMSequencer. The transcriptome data were processed and compared with reference genome, differential expression analysis, GO and KEGG enrichment analysis and candidate gene screening. Real-time PCR method was used to detect the reliability of transcriptional data. The results showed that the unique alignment rates of 86 954 060 and 69 837 009 reads; in winter and summer were 73.21 and 75.55, respectively. There were 182 differentially expressed genes (DEGs), in winter and summer groups, 117 of which were up-regulated in winter and 65 down-regulated in winter. Real-time PCR analysis showed that the sequencing of transcriptome was reliable. GO analysis showed that cell components, molecular functions and biological processes were enriched to 21n39 and 224 term;, respectively. KEGG analysis showed that the differentially expressed gene (DEGs) was significantly enriched in lipid metabolism, coagulation, tyrosine metabolism, steroid synthesis and retinol metabolism, and 8 candidate genes were screened out. In conclusion, lipid metabolism, coagulation, melanin synthesis related pathways and genes, as well as the genes related to hair development, may play an important role in the cold resistance of Mongolian cattle.
【作者单位】：内蒙古农业大学生命科学学院;
【基金】：国家科技支撑计划项目(2011BAD18B00) 内蒙古自治区科技计划项目(20131403)
【分类号】：S823.81

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