PRRSV上海浦东南汇分离株Nsp2与ORF5基因遗传演化分析

发布时间：2018-11-10 18:43

【摘要】：对上海浦东南汇地区某规模猪场猪繁殖与呼吸综合征病毒(PRRSV)疑似病料进行处理,提取病毒总RNA,采用RT-PCR方法扩增Nsp2/ORF5基因,并进行基因测序。序列分析结果表明,浦东南汇分离株Nsp2蛋白均缺失481位和第533~561位30个不连续的氨基酸,同时在Nsp2编码区出现与高致病性PRRSV(HP-PRRSV)代表株JXA1相同的遗传变异现象。同源性分析表明,6株Nsp2、ORF5氨基酸序列与VR-2332、CH-1a、HB-2以及JXA1株的同源性分别为:40.3%~55.3%,73.5%~89.5%;54.7%~75.1%,75.5%~92.5%;50.9%~67.3%,73.5%~90.5%;72.8%~91.5%,81.5%~98.5%;而与欧洲型代表株LV株的同源性分别仅为26.2%~35.9%和44.8%~56.4%。遗传演化分析表明,浦东南汇分离株与国内株(CH-1a、HB-2)和VR-2332美洲代表株各自处于相对独立的分支,遗传关系较远且不存在交叉现象,而与JXA1变异株处于同一进化分支。研究表明,浦东南汇分离株为美洲型HP-PRRSV毒株,具有一定的地理衍变特性,已从PRRSV遗传进化树中分离,出现一个新的遗传独立群体。
[Abstract]:The suspected (PRRSV) of porcine reproductive and respiratory syndrome virus (RRRSV) was treated in a pig farm in Nanhui area, Pudong, Shanghai. The total RNA, of the virus was extracted and the Nsp2/ORF5 gene was amplified by RT-PCR method, and the gene was sequenced. The results of sequence analysis showed that the Nsp2 protein of Pudong Nanhui isolate was short of 30 discontinuous amino acids at position 581 and 533 ~ (561), and the same genetic variation occurred in the coding region of Nsp2 as that of JXA1, a representative strain of highly pathogenic PRRSV (HP-PRRSV). Homology analysis showed that the amino acid sequences of 6 Nsp2,ORF5 strains were 40.3% and 73.53.5% with VR-2332,CH-1a,HB-2 and JXA1 strains, respectively. 54.7 including 75.1 and 75.5, including 92.5 and 50.9 and 67.3, and 73.5 and 72.5, and 91.5 and 98.5, as well as 91.5 and 98.5. The homology with LV strain was only 26.235.9% and 44.8%, respectively. Genetic evolution analysis showed that the isolates of Pudong South Huihui were relatively independent of each other with CH-1a,HB-2 and VR-2332 representative strains, and the genetic relationship was far away and there was no cross phenomenon. However, it was in the same evolutionary branch as the JXA1 mutant. The results show that the isolated strain of HP-PRRSV in Pudong South Huihui is of American type and has certain geographical evolution characteristics. It has been isolated from the genetic evolution tree of PRRSV and a new genetic independent population has emerged.
【作者单位】：上海浦东新区农业服务中心南汇新城镇站;上海浦东新区农业服务中心书院站;上海闵行区动物疫病预防控制中心;扬州大学兽医学院;
【基金】：上海市科技兴农推广项目[沪农科推字(2015)第1-5号]
【分类号】：S852.651

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