结缕草ZjCSD基因的克隆及表达分析

发布时间：2018-11-10 19:56

【摘要】：铜锌超氧化物歧化酶是植物响应逆境胁迫过程中的关键酶,其含量和活性与植物抗逆性密切相关。本研究以结缕草cDNA为模板,利用同源克隆法,从结缕草转录组数据库中克隆获得了结缕草ZjCSD基因,该基因编码一个含有152个氨基酸的蛋白质。生物信息学分析结果显示:ZjCSD基因编码蛋白为稳定的、亲水的、酸性、非分泌脂溶蛋白,定位于细胞质中,含有CSD蛋白家族特有的保守结构域,具有典型的Cu~(2+)和Zn~(2+)结合位点;与小米、玉米等禾本科植物具有较高的同源性,进化关系较近。采用实时荧光定量PCR研究该基因在不同组织中、不同胁迫处理下的表达模式,结果表明,ZjCSD基因在根、茎、叶中都有表达,叶中表达量最高;干旱胁迫(30%PEG)、盐胁迫(150mmol/L NaCl)和Cd~(2+)胁迫(200mg/L Cd~(2+))均能诱导ZjCSD基因表达量上调,Pb~(2+)胁迫(1g/L Pb~(2+))诱导ZjCSD基因表达量下调。故推测结缕草ZjCSD基因在结缕草应对干旱、盐和重金属胁迫的过程中发挥作用。
[Abstract]:Copper-zinc superoxide dismutase (SOD) is a key enzyme in plant response to stress, and its content and activity are closely related to plant stress resistance. In this study, the ZjCSD gene of Zoysia japonica was cloned from Zoysia japonica transcriptional database by homologous cloning using cDNA as template. The gene encodes a protein containing 152 amino acids. Bioinformatics analysis showed that the protein encoded by ZjCSD gene was stable, hydrophilic, acidic and non-secreted adiponectin, located in the cytoplasm and containing the conserved domain of CSD protein family. There are typical Cu~ (2) and Zn~ (2) binding sites. It has high homology with millet, corn and other gramineous plants, and its evolutionary relationship is close. Real-time fluorescence quantitative PCR was used to study the expression patterns of the gene in different tissues and under different stresses. The results showed that ZjCSD gene was expressed in roots, stems and leaves, and the highest expression was found in leaves. Drought stress (30%PEG), salt stress (150mmol/L NaCl) and Cd~ (2) stress (200mg/L Cd~ (2) could induce the up-regulation of ZjCSD gene expression, and Pb~ (2) stress (1g/L Pb~ (2) could induce the down-regulation of ZjCSD gene expression. It is suggested that ZjCSD gene plays an important role in drought, salt and heavy metal stress of Zoysia japonica.
【作者单位】：北京林业大学草坪研究所;河北省作物生长调控重点实验室河北农业大学;
【基金】：中国林学会——青年人才托举工程项目国家高新技术研究发展计划项目(2013AA102607)资助
【分类号】：S688.4

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