食源性单核增生性李斯特氏菌毒力基因的分布

发布时间：2018-11-11 00:02

【摘要】：为探明保定、石家庄及周边地区食源性单增李斯特菌的污染状况以及食源性单增李斯特菌中毒力基因的分布情况,从保定、石家庄及周边地区农贸市场、超市采集七大类食品,共1 288个样品进行单增李斯特菌的污染调查,对鉴定分离出的308株食源性单增李斯特菌的27个毒力基因进行PCR检测。结果表明:七大类食品中单增李斯特菌的平均检出率为23.91%,动物源食品单增李斯特菌污染远高于植物源食品;27个毒力基因的PCR检测发现,inl、plcA、plcB、hpt、hly及fbp等与侵入、感染有关的毒力基因在动物源食品分离株中分布明显高于植物源食品分离株;而iap、ami、dlt、prfA、virR、hfq与粘附、调控有关的毒力基因在动物源食品分离株和在植物源食品分离株中差别不明显;hfq、dltC和iap 3个基因的检出率最高,分别达到94.68%,93.36%和91.03%,可作为该地区食源性单增李斯特氏菌鉴定的参考基因。
[Abstract]:In order to find out the contamination status of Listeria monocytogenes in Baoding, Shijiazhuang and its surrounding areas and the distribution of toxic genes of Listeria monocytogenes, seven kinds of food were collected from Baoding, Shijiazhuang and surrounding farmers' markets and supermarkets. A total of 1 288 samples were investigated for the contamination of Listeria monocytogenes, and 27 virulence genes of 308 isolated strains of Listeria monocytogenes were detected by PCR. The results showed that the average detection rate of Listeria monocytogenes in seven kinds of food was 23.91. The contamination of Listeria monocytogenes in animal food was much higher than that in plant-based food. PCR analysis of 27 virulence genes showed that the distribution of virulence genes related to invasion and infection of inl,plcA,plcB,hpt,hly and fbp in animal food isolates was significantly higher than that in plant food isolates. However, the virulence genes related to adhesion and regulation of iap,ami,dlt,prfA,virR,hfq were not significantly different between animal food isolates and plant food isolates. The detection rate of hfq,dltC and iap were the highest (94.68% 93.36% and 91.03% respectively), which could be used as reference genes for identification of Listeria monocytogenes.
【作者单位】：河北农业大学食品科技学院;河北科技大学生物科学与工程学院;
【基金】：河北省自然科学基金项目(No.C2006000514)
【分类号】：TS201.3

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