自然杀伤细胞免疫球蛋白样受体基因与乳腺癌的相关性分析

发布时间：2018-11-11 00:20

【摘要】：为了探讨自然杀伤(natural killer,NK)细胞免疫球蛋白样受体基因KIRs与乳腺癌发生发展的关系,我们以45例乳腺癌患者为研究组,30例健康成年女性为对照组,分别采集其血液样本进行聚合酶链式反应(polymerase chain reaction,PCR),对两组中自然杀伤细胞免疫球蛋白样受体基因簇成员与其配体及其相关组合的表达频率进行了比较分析,发现与对照组相比,乳腺癌患者中KIR2DL5A、KIR2DS2和KIR2DS3 3种基因表达的频率显著降低,与乳癌症的发生发展成负相关关系,而KIR2DL1与KIR3DS1基因的频率稍高,但无统计学意义。基因的组合分析显示,KIR2DS2和KIR2DS3对乳腺癌患者具有一定的保护作用,在KIR2DS2缺失时KIR2DL2可成为危险因素。乳腺癌患者组内HLA-C1的基因型(93.3%)显著高于对照组(73.3%),抑制性的基因组合KIR2DL1+/HLA-C1C2+与乳腺癌之间存在正性相关关系;对于同一种KIR基因,负性相关关系见于KIR2DL1+/C2+(p0.001)及KIR2DL1+/HLA-C2C2+(p=0.022)组合。我们的研究为了解乳腺癌的免疫遗传机制及其临床诊疗提供了一定的帮助。
[Abstract]:In order to investigate the relationship between natural killer (natural killer,NK) cell immunoglobulin-like receptor gene (KIRs) and the occurrence and development of breast cancer, we studied 45 breast cancer patients and 30 healthy adult women as control group. Blood samples were collected for polymerase chain reaction (polymerase chain reaction,PCR), and the expression frequency of the members of natural killer cell immunoglobulin-like receptor gene cluster, its ligands and their related combinations in two groups were compared and analyzed. It was found that the frequency of KIR2DL5A,KIR2DS2 and KIR2DS3 gene expression in breast cancer patients was significantly lower than that in the control group, which was negatively correlated with the occurrence and development of breast cancer, while the frequency of KIR2DL1 and KIR3DS1 gene was slightly higher, but had no statistical significance. Gene combination analysis showed that KIR2DS2 and KIR2DS3 had a protective effect on breast cancer patients, and KIR2DL2 could be a risk factor when KIR2DS2 was absent. The genotype of HLA-C1 in breast cancer group (93.3%) was significantly higher than that in control group (73.3%). There was a positive correlation between inhibitory gene combination KIR2DL1 / HLA-C1C2 and breast cancer. For the same KIR gene, the negative correlation was found in the combination of KIR2DL1 / C2 (p0.001) and KIR2DL1 / HLA-C2C2 (p0. 022). Our research is helpful to understand the immunogenetic mechanism of breast cancer and its clinical diagnosis and treatment.
【作者单位】：潍坊市益都中心医院;泰山医学院附属医院;
【基金】：基金项目(No.31371504)资助
【分类号】：R737.9

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