青海地区产ESBLs大肠埃希菌,肺炎克雷伯菌基因分型研究

发布时间：2018-11-11 16:00

【摘要】：目的:明确青海地区产ESBLs大肠埃希菌,肺炎克雷伯菌主要基因型的分布特点,为临床合理用药提供依据。方法:收集青海5家医院产ESBLs大肠埃希菌和肺炎克雷伯菌共233株(大肠埃希菌189株,肺炎克雷伯菌44株)。采用美国临床实验室标准化委员会规定的ESBL表型筛选和确证试验确定ESBL的发生率、PCR扩增对产ESBL菌初步分型,扩增阳性产物测序和分析,进一步确定基因型。结果:共筛选出208株产ESBLs菌株,检出携带β-内酰胺酶基因菌株128株,基因型别10种,占总样本的61.54%,其中单基因型别92株(CTX-M型68株,TEM-1型24株),占总样本数的44.23%;双基因型别34株,(CTX-M合并TEM-1型32株,两种不同亚型的CTX-M共存2株),占总样本数的16.35%;三基因型别2株,为两种不同亚型的CTX-M合并TEM-1型,占总样本数0.96%。结论:青海地区产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和肺炎克雷伯菌主要基因型为CTX-M型和TEM-1型。
[Abstract]:Objective: to identify the distribution characteristics of major genotypes of Escherichia coli and Klebsiella pneumoniae produced in Qinghai, and to provide evidence for rational drug use. Methods: 233 strains of Escherichia coli and Klebsiella pneumoniae were collected from 5 hospitals in Qinghai province (189 strains of Escherichia coli and 44 strains of Klebsiella pneumoniae). The incidence of ESBL was determined by the ESBL phenotypic screening and confirmatory test prescribed by the American Clinical Laboratory Standardization Committee. The ESBL producing bacteria were preliminarily typed by PCR amplification and the positive products were sequenced and analyzed to further determine the genotype. Results: a total of 208 ESBLs producing strains were screened out, 128 strains with 尾 -lactamase gene were detected, 10 genotypes were identified, accounting for 61.54% of the total sample, among which 92 strains were single-genotype (68 CTX-M type, 24 TEM-1 type). 44.23% of the total sample number; There were 34 double genotypes (32 CTX-M combined with TEM-1, 2 CTX-M coexisting with two different subtypes), accounting for 16.35% of the total samples. The three genotypes were two different subtypes of CTX-M combined with TEM-1, accounting for 0.96% of the total samples. Conclusion: the major genotypes of extended-spectrum 尾 -lactamase producing Escherichia coli and Klebsiella pneumoniae in Qinghai are CTX-M and TEM-1.
【作者单位】：青海省西宁市第一人民医院检验科;
【分类号】：R440

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