华南常用籼稻亲本稻瘟病抗性评价及抗性基因鉴定

发布时间：2018-11-12 16:24

【摘要】：【目的】评价分析华南地区常用籼稻亲本稻瘟病抗性及抗性基因,为水稻稻瘟病抗性育种提供理论依据。【方法】利用人工接种鉴定对52份华南地区常用籼稻亲本进行稻瘟病抗性分析,同时利用分子标记检测稻株携带的抗性基因,分析亲本材料稻瘟病抗性与其所携带稻瘟病抗性基因间的关系。【结果】从52份适应华南生态条件的籼稻亲本材料中筛选获得4份广谱抗源(2份抗谱为100.0%,2份抗谱为92.9%);常规稻较恢复系和保持系具有更高的稻瘟病抗性。ZA1、ZA9和ZB9生理小种较ZB1和ZB5生理小种表现出更强的致病力,具有较低的抗性频率。Pi-ta和Pi54基因的出现频率最高,均为0.42;其次是Pib基因,出现频率为0.37;Pi-km基因的出现频率较低,仅0.06;所有籼稻亲本材料中均未检测出Pi9基因。携带Pib+Pi54+Pi-km和Pi-ta+Pib+Pi54三基因稻株的抗性高于所有单基因或双基因稻株。【结论】华南常用籼稻亲本材料中存在着优良的稻瘟病广谱抗源,可利用这些抗源拓宽育种材料的遗传背景,培育抗病优良品种。多个抗性基因聚合能有效提高稻株对稻瘟病不同生理小种的抗性,在育种实践中应加强多基因聚合材料的创制和利用研究。
[Abstract]:[objective] to evaluate and analyze the resistance and resistance genes of common indica rice parents to rice blast in South China. [methods] the resistance of 52 common indica rice parents in South China was analyzed by artificial inoculation, and the resistance genes of rice plants were detected by molecular markers. The relationship between rice blast resistance and rice blast resistance gene was analyzed. [results] four broad-spectrum resistance sources were obtained from 52 indica rice parents adapted to the ecological conditions in South China. The resistance spectrum of 2 samples was 92.9%. Compared with restorer line and maintainer line, conventional rice had higher resistance to blast, ZA1,ZA9 and ZB9 showed stronger pathogenicity and lower resistance frequency than ZB1 and ZB5. Pi-ta and Pi54 gene appeared the highest frequency. All of them were 0.42; The second was Pib gene, the frequency of which was 0.37 Pi-km gene, the frequency was only 0.06.The Pi9 gene was not detected in all indica rice parents. The resistance of rice plants carrying Pib Pi54 Pi-km and Pi-ta Pib Pi54 genes was higher than that of all single-gene or double-gene rice plants. [conclusion] there are excellent broad-spectrum resistance sources of rice blast in common indica rice parents in South China. These resistance sources can be used to broaden the genetic background of breeding materials and to cultivate good varieties of disease resistance. Multi-resistance gene polymerization can effectively improve the resistance of rice plants to different physiological races of rice blast. In breeding practice, the creation and utilization of polygenic polymeric materials should be strengthened.
【作者单位】：广西农业科学院水稻研究所/广西水稻遗传育种重点实验室/国家水稻改良中心南宁分中心;广西农业科学院农业科技信息研究所;广西农业科学院植物保护研究所;
【基金】：国家“863”计划项目(2014AA10A604) 国家自然科学基金项目(31360329) 广西农业科学院基本科研业务专项项目(桂农科2016YM59,桂农科2015YM10)
【分类号】：S435.111.41

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